Please use this identifier to cite or link to this item:
Title: Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
Author: Sebastián Muñoz, David
Guitart, Maria
García Martínez, Celia
Mauvezin, Caroline
Orellana Gavaldà, Josep Maria
Serra i Cucurull, Dolors
Gómez Foix, Anna Maria
García Hegardt, Fausto
Asins Muñoz, Guillermina
Keywords: Metabolisme
Àcids grassos
Reacció d'oxidació-reducció
Cèl·lules musculars
Metabolisme dels lípids
Ratolins (Animals de laboratori)
Fatty acids
Oxidation-reduction reaction
Muscle cells
Lipid metabolism
Mice (Laboratory animals)
Issue Date: Sep-2009
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may interact with CPT1 and contribute to fatty acid import into mitochondria in muscle. Here, we demonstrate that another membrane-bound fatty acid binding protein, fatty acid transport protein 1 (FATP1), collaborates with CPT1 for fatty acid import into mitochondria. Overexpression of FATP1 using adenovirus in L6E9 myotubes increased both fatty acid oxidation and palmitate esterification into triacylglycerides. Moreover, immunocytochemistry assays in transfected L6E9 myotubes showed that FATP1 was present in mitochondria and coimmunoprecipitated with CPT1 in L6E9 myotubes and rat skeletal muscle in vivo. The cooverexpression of FATP1 and CPT1 also enhanced mitochondrial fatty acid oxidation, similar to the cooverexpression of FAT/CD36 and CPT1. However, etomoxir, an irreversible inhibitor of CPT1, blocked all these effects. These data reveal that FATP1, like FAT/CD36, is associated with mitochondria and has a role in mitochondrial oxidation of fatty acids.
Note: Reproducció del document publicat a:
It is part of: Journal of Lipid Research, 2009, vol. 50, num. 9, p. 1789-1799
Related resource:
ISSN: 0022-2275
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Files in This Item:
File Description SizeFormat 
579374.pdf1.18 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.