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http://hdl.handle.net/2445/165174
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DC Field | Value | Language |
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dc.contributor.author | Pino Saladrigues, Marta del | - |
dc.contributor.author | Martí Delgado, Cristina | - |
dc.contributor.author | Gaber, Jina | - |
dc.contributor.author | Svanholm-Barrie, Cecilia | - |
dc.contributor.author | Rodríguez-Carunchio, Leonardo | - |
dc.contributor.author | Rodríguez Trujillo, Adriano | - |
dc.contributor.author | Carreras, Núria | - |
dc.contributor.author | Fuertes, Irene | - |
dc.contributor.author | Barnadas, Esther | - |
dc.contributor.author | Marimon, Lorena | - |
dc.contributor.author | Blanco, José L. | - |
dc.contributor.author | Persing, David H. | - |
dc.contributor.author | Torné Bladé, Aureli | - |
dc.contributor.author | Ordi i Majà, Jaume | - |
dc.date.accessioned | 2020-06-11T15:41:49Z | - |
dc.date.available | 2020-06-11T15:41:49Z | - |
dc.date.issued | 2019-11-02 | - |
dc.identifier.issn | 2075-4418 | - |
dc.identifier.uri | http://hdl.handle.net/2445/165174 | - |
dc.description.abstract | There is growing interest in anal cancer screening strategies. However, cytological/molecular evaluation of anal samples is challenging. We aimed to determine the feasibility of detecting, in anal liquid-based cytologies, the expression of biomarkers involved in the cell cycle disturbance elicited by human papillomavirus (HPV). The accuracy of this approach in the identification of high-grade squamous intraepithelial lesions/anal intraepithelial neoplasia grade2-3 (HSIL/AIN2-3) was also evaluated. 215 anal cytologies from men having sex with men living with human immunodeficiency virus were evaluated. Patients showing concordant cytological and anoscopy-directed biopsy diagnosis were selected: 70 with negative cytology and HPV test, 70 with low-grade SIL (LSIL/AIN1) cytology and biopsy, and 75 with cytology and biopsy of HSIL/AIN2-3. CDKN2A/p16, MKI67 and TOP2A mRNA expression was analyzed. HPV detection was performed with Xpert HPV Assay (Cepheid, Sunnyvale, CA, USA). HSIL/AIN2-3 showed higher expression for the biomarkers than LSIL/AIN1 or negative samples. The specificity for HSIL/AIN2-3 detection for a sensitivity established at 70% was 44.7% (95%confidence interval [CI] 36.5-53.2) for TOP2A and MKI67 and 54.5% (95%CI 46.0-62.8%) for CDKN2A/p16. mRNA detection of cell biomarkers in anal liquid-based cytology is feasible. Further studies are warranted to confirm if strategies based on mRNA detection have any role in anal cancer screening. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/diagnostics9040173 | - |
dc.relation.ispartof | Diagnostics, 2019, vol. 9, num. 4, p. 191 | - |
dc.relation.uri | https://doi.org/10.3390/diagnostics9040173 | - |
dc.rights | cc-by (c) Pino Saladrigues, Marta del et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) | - |
dc.subject.classification | Càncer colorectal | - |
dc.subject.classification | Citologia | - |
dc.subject.classification | RNA | - |
dc.subject.classification | Marcadors bioquímics | - |
dc.subject.other | Colorectal cancer | - |
dc.subject.other | Cytology | - |
dc.subject.other | RNA | - |
dc.subject.other | Biochemical markers | - |
dc.title | mRNA Detection in Anal Cytology: A Feasible Approach for Anal Cancer Screening in Men Who Have Sex with Men Living With HIV | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 695640 | - |
dc.date.updated | 2020-06-11T15:41:49Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31684110 | - |
Appears in Collections: | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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695640.pdf | 1.37 MB | Adobe PDF | View/Open |
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