Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/167702
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, Su | - |
dc.contributor.author | Keung, Wendy | - |
dc.contributor.author | Serra i Cucurull, Dolors | - |
dc.contributor.author | Wang, Wei | - |
dc.contributor.author | Carrasco, Patricia | - |
dc.contributor.author | Casals i Farré, Núria | - |
dc.contributor.author | Hegardt, Fausto | - |
dc.contributor.author | Moran, Timothy H. | - |
dc.contributor.author | Lopaschuk, Gary D. | - |
dc.date.accessioned | 2020-07-06T07:48:32Z | - |
dc.date.available | 2020-07-06T07:48:32Z | - |
dc.date.issued | 2011-04-20 | - |
dc.identifier.issn | 0363-6119 | - |
dc.identifier.uri | http://hdl.handle.net/2445/167702 | - |
dc.description.abstract | Hypothalamic fatty acid metabolism is involved in central nervous system controls of feeding and energy balance. Malonyl-CoA, an intermediate of fatty acid biosynthesis, is emerging as a significant player in these processes. Notably, hypothalamic malonyl-CoA has been implicated in leptin's feeding effect. Leptin treatment increases malonyl-CoA level in the hypothalamic arcuate nucleus (Arc), and this increase is required for leptin-induced decrease in food intake. However, the intracellular downstream mediators of malonyl-CoA's feeding effect have not been identified. A primary biochemical action of malonyl-CoA is the inhibition of the acyltransferase activity of carnitine palmitoyltransferase-1 (CPT-1). In the hypothalamus, the predominant isoform of CPT-1 that possesses the acyltransferase activity is CPT-1 liver type (CPT-1a). To address the role of CPT-1a in malonyl-CoA's anorectic action, we used a recombinant adenovirus expressing a mutant CPT-1a that is insensitive to malonyl-CoA inhibition. We show that Arc overexpression of the mutant CPT-1a blocked the malonyl-CoA-mediated inhibition of CPT-1 activity. However, the overexpression of this mutant did not affect the anorectic actions of leptin or central cerulenin for which an increase in Arc malonyl-CoA level is also required. Thus, CPT-1a does not appear to be involved in the malonyl-CoA's anorectic actions induced by leptin. Furthermore, long-chain fatty acyl-CoAs, substrates of CPT-1a, dissociate from malonyl-CoA's actions in the Arc under different feeding states. Together, our results suggest that Arc intracellular mechanisms of malonyl-CoA's anorectic actions induced by leptin are independent of CPT-1a. The data suggest that target(s), rather than CPT-1a, mediates malonyl-CoA action on feeding. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Physiological Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1152/ajpregu.00092.2011 | - |
dc.relation.ispartof | American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 2011, vol. 301, num. 1, p. R209-17 | - |
dc.relation.uri | https://doi.org/10.1152/ajpregu.00092.2011 | - |
dc.rights | (c) American Physiological Society, 2011 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Fisiologia | - |
dc.subject.classification | Genètica | - |
dc.subject.classification | Mutació (Biologia) | - |
dc.subject.classification | Alimentació | - |
dc.subject.other | Physiology | - |
dc.subject.other | Genetics | - |
dc.subject.other | Mutation (Biology) | - |
dc.subject.other | Diet | - |
dc.title | Malonyl-CoA Mediates Leptin Hypothalamic Control of Feeding Independent of Inhibition of CPT-1a | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 597270 | - |
dc.date.updated | 2020-07-06T07:48:32Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 21508288 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
597270.pdf | 273.23 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.