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http://hdl.handle.net/2445/167757
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DC Field | Value | Language |
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dc.contributor.author | González, Juan D. | - |
dc.contributor.author | Silva-Marrero, Jonás I. | - |
dc.contributor.author | Metón Teijeiro, Isidoro | - |
dc.contributor.author | Caballero Solares, Albert | - |
dc.contributor.author | Viegas, Iván | - |
dc.contributor.author | Fernández González, Felipe Javier | - |
dc.contributor.author | Miñarro Carmona, Montserrat | - |
dc.contributor.author | Fábregas, A. (Anna) | - |
dc.contributor.author | Ticó Grau, Josep R. | - |
dc.contributor.author | Jones, John G. | - |
dc.contributor.author | Vázquez Baanante, Ma. Isabel | - |
dc.date.accessioned | 2020-07-06T08:55:06Z | - |
dc.date.available | 2020-07-06T08:55:06Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 1436-2228 | - |
dc.identifier.uri | http://hdl.handle.net/2445/167757 | - |
dc.description.abstract | Alanine aminotransferase (ALT) catalyses a transamination reaction that links carbohydrate and amino acid metabolism. In this study, we examined the effect of silencing cytosolic ALT (cALT) expression on the hepatic metabolism in Sparus aurata. A number of siRNA and shRNA designed to down-regulate cALT expression were validated in HEK-293 cells transfected with plasmids expressing S. aurata cALT or mitochondrial ALT (mALT) isoforms: ALT silencing significantly decreased the expression levels of S. aurata mRNA cALT1 to 62 % (siRNA) and 48 % (shRNA) of the values observed in control cells. The effect of cALT silencing was analysed in the liver of S. aurata 72 h after intraperitoneal injection of chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid encoding a shRNA to down-regulate cALT expression (pCpG-si1sh1). In fish fed diets with different ratio of protein to carbohydrate and treated with chitosan-TPP-pCpG-si1sh1, cALT1 and cALT2 mRNA levels significantly decreased irrespective of the diet. Consistently, ALT activity decreased in liver of treated animals. In the liver of S. aurata treated with chitosan-TPP-pCpG-si1sh1 nanoparticles, down-regulation of cALT expression increased the activity of key enzymes in glycolysis (6-phosphofructo-1-kinase and pyruvate kinase) and protein metabolism (glutamate dehydrogenase). Besides showing for the first time that administration of chitosan-TPP-pCpG-si1sh1 nanoparticles silences hepatic cALT expression in vivo, our data support that down-regulation of cALT could improve the use of dietary carbohydrates to obtain energy and spare protein catabolism | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Verlag | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1007/s10126-015-9670-8 | - |
dc.relation.ispartof | Marine Biotechnology, 2016, vol. 18, num. 1, p. 85-97 | - |
dc.relation.uri | https://doi.org/10.1007/s10126-015-9670-8 | - |
dc.rights | (c) Springer Verlag, 2016 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Quitosan | - |
dc.subject.classification | Teràpia genètica | - |
dc.subject.classification | Orada | - |
dc.subject.classification | Nanopartícules | - |
dc.subject.classification | Metabolisme | - |
dc.subject.other | Chitosan | - |
dc.subject.other | Gene therapy | - |
dc.subject.other | Sparus aurata | - |
dc.subject.other | Nanoparticles | - |
dc.subject.other | Metabolism | - |
dc.title | Chitosan-mediated shRNA knockdown of cytosolic alanine aminotransferase improves hepatic carbohydrate metabolism | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 654653 | - |
dc.date.updated | 2020-07-06T08:55:06Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
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654653.pdf | 2.67 MB | Adobe PDF | View/Open |
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