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Title: Targeted proteomics in urinary extracellular vesicles identifies biomarkers for diagnosis and prognosis of prostate cancer
Author: Sequeiros, Tamara
Rigau, Marina
Chiva, Cristina
Montes, Melania
Garcia-Grau, Iolanda
Garcia, Marta
Diaz, Sherley
Celma, Ana
Bijnsdorp, Irene
Campos, Alex
Mauro, Primiano Di
Borrós, Salvador
Reventós Puigjaner, Jaume
Doll, Andreas
Paciucci Barzanti, Rosanna
Pegtel, D. Michiel
Torres, Inés de
Sabidó Aguadé, Eduard
Morote, Juan
Olivan Riera, Mireia
Keywords: Indicadors biològics
Càncer de pròstata
Indicators (Biology)
Prostate cancer
Issue Date: 17-Jan-2017
Publisher: Impact Journals
Abstract: Rapid and reliable diagnosis of prostate cancer (PCa) is highly desirable as current used methods lack specificity. In addition, identification of PCa biomarkers that can classify patients into high- and low-risk groups for disease progression at early stage will improve treatment decision-making. Here, we describe a set of protein-combination panels in urinary extracellular vesicles (EVs), defined by targeted proteomics and immunoblotting techniques that improve early non-invasive detection and stratification of PCa patients.We report a two-protein combination in urinary EVs that classifies benign and PCa patients (ADSV-TGM4), and a combination of five proteins able to significantly distinguish between high- and low-grade PCa patients (CD63-GLPK5-SPHM-PSA-PAPP). Proteins composing the panels were validated by immunohistochemistry assays in tissue microarrays (TMAs) confirming a strong link between the urinary EVs proteome and alterations in PCa tissues. Moreover, ADSV and TGM4 abundance yielded a high diagnostic potential in tissue and promising TGM4 prognostic power. These results suggest that the proteins identified in urinary EVs distinguishing high- and low grade PCa are a reflection of histological changes that may be a consequence of their functional involvement in PCa development. In conclusion, our study resulted in the identification of protein-combination panels present in urinary EVs that exhibit high sensitivity and specificity for PCa detection and patient stratification. Moreover, our study highlights the potential of targeted proteomic approaches-such as selected reaction monitoring (SRM)-as diagnostic assay for liquid biopsies via urinary EVs to improve diagnosis and prognosis of suspected PCa patients.
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It is part of: Oncotarget, 2017, vol. 8, num. 3, p. 4960-4976
Related resource:
ISSN: 1949-2553
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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