Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/168023
Title: | A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis |
Author: | Rapado González, Óscar Majem, Blanca Álvarez Castro, Ana Díaz Peña, Roberto Abalo, Alicia Suárez Cabrera, Leticia Gil Moreno, Antonio Santamaría, Anna López López, Rafael Muinelo Romay, Laura Suarez Cunqueiro, María Mercedes |
Keywords: | Càncer colorectal Marcadors bioquímics Colorectal cancer Biochemical markers |
Issue Date: | 1-Dec-2019 |
Publisher: | Mdpi |
Abstract: | Salivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oral cancer. However, little is known about their value for colorectal cancer (CRC) detection. Our study aims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis. The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthy controls. The differential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomas and 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regression models were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAs were significantly deregulated in CRC patients vs. healthy individuals (p < 0.05) in the discovery phase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, and miR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (p < 0.05). This five-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754) to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels. Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel was identified as a promising tool to diagnose this malignancy, representing a novel approach to detect cancer-associated epigenetic alterations using a non-invasive strategy. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/jcm8122029 |
It is part of: | Journal Of Clinical Medicine, 2019-12-01, Vol. 8, Issue 12 |
URI: | http://hdl.handle.net/2445/168023 |
Related resource: | https://doi.org/10.3390/jcm8122029 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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Rapado-GonzalezO.pdf | 2.2 MB | Adobe PDF | View/Open |
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