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http://hdl.handle.net/2445/168223
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DC Field | Value | Language |
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dc.contributor.author | Martínez Valenzuela, Laura | - |
dc.contributor.author | Draibe, Juliana | - |
dc.contributor.author | Quero Ramos, Maria | - |
dc.contributor.author | Fulladosa, Xavier | - |
dc.contributor.author | Cruzado, Josep Ma. | - |
dc.contributor.author | Bestard Matamoros, Oriol | - |
dc.contributor.author | Torras Ambròs, Joan | - |
dc.date.accessioned | 2020-07-09T11:07:56Z | - |
dc.date.available | 2020-07-09T11:07:56Z | - |
dc.date.issued | 2019-11-20 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/2445/168223 | - |
dc.description.abstract | Background: the role of the T helper 17 (Th17) cell subset in anti-neutrophil cytoplasm antibodies (ANCA) associated vasculitis (AAV) is controversial. We hypothesized that a specific Th17 response to myeloperoxidase (MPO) or proteinase 3 (PR3) is detectable in AAV patients and is different among the disease phases. Methods: we analyzed 43 AAV patients with renal involvement (21 acute and 22 remission patients), and 12 healthy controls. Peripheral blood mononuclear cells (PBMCs) were cultured with PR3/MPO over 48 h. Thereafter, frequencies of MPO/PR3-specific Th17 cells were assessed using an enzyme-linked immunosorbent spot (ELISpot) assay. Supernatant IL-17 concentration was quantified using ELISA. Finally, specific Th17 response after depletion of T regulatory lymphocytes (T-regs) in some remission patients was compared to the non T-reg-depleted response. Results: specific Th17 cell number was higher in acute patients compared to remission (p = 0.004). Specific Th17 cell number performed well in the disease activity detection (ROC curve area under the curve (AUC) = 0.87; p = 0.0001) with an optimal cut-off of 6 spots/million. Patients above this cut-off showed higher serum creatinine (p = 0.004), C-reactive protein (CRP) (p = 0.001) and ANCA titer (p = 0.032). Supernatant IL-17 concentration was higher in acute patients compared to remission (p = 0.035) and did not normalize to healthy control levels (p = 0.01). Conclusions: a specific Th17 cell response is present in AAV patients. This response is more pronounced in the acute phase, but persists in remission. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms20235820 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2019, vol. 20, num. 23, p. 5820 | - |
dc.relation.uri | https://doi.org/10.3390/ijms20235820 | - |
dc.rights | cc-by (c) Martínez Valenzuela, Laura et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Vasculitis | - |
dc.subject.classification | Diagnòstic | - |
dc.subject.classification | Cèl·lules | - |
dc.subject.classification | Immunologia | - |
dc.subject.other | Vasculitis | - |
dc.subject.other | Diagnosis | - |
dc.subject.other | Cells | - |
dc.subject.other | Immunology | - |
dc.title | Exploring frequencies of circulating specific Th17 cells against myeloperoxidase and proteinase 3 in ANCA associated vasculitis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 700039 | - |
dc.date.updated | 2020-07-09T11:07:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31756913 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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700039.pdf | 1.7 MB | Adobe PDF | View/Open |
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