Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/168441
Title: | A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics |
Author: | Berbel, Dámaris Cámara, Jordi García, Ernesto Tubau, Fe Guérin, François Giard, Jean-Christophe Domínguez Luzón, Ma. Ángeles (María Ángeles) Cattoir, Vincent Ardanuy Tisaire, María Carmen |
Keywords: | Fenotip Estreptococs Antibiòtics Phenotype Streptococcus Antibiotics |
Issue Date: | 1-Nov-2019 |
Publisher: | Elsevier B.V. |
Abstract: | A lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes [group A streptococcus (GAS)]. The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics. Antimicrobial susceptibility was tested using the microdilution broth method and the resistance phenotype was tested by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). Whole-genome sequencing revealed that the strain was ST10 and emm93, and had five resistance genes [lnu(B), ant(6)-Ia, aph(3')-III, tet(M) and dfrG]. The tet(M) gene was located in a Tn916-like transposon. The lsa(E)-lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Ia-sat4-aph(3')III] and lsa(E)-lnu(B) genes. This structure was not transferred by conjugation. This study identified a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.ijantimicag.2019.08.019 |
It is part of: | International Journal of Antimicrobial Agents, 2019, vol. 54, num. 5, p. 647-651 |
URI: | http://hdl.handle.net/2445/168441 |
Related resource: | https://doi.org/10.1016/j.ijantimicag.2019.08.019 |
ISSN: | 0924-8579 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
693755.pdf | 449.61 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License