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DC Field | Value | Language |
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dc.contributor.author | Miñana i Galbis, David | - |
dc.contributor.author | Urbizu Serrano, Aintzane | - |
dc.contributor.author | Farfán Sellarés, Maribel | - |
dc.contributor.author | Fusté Munné, M. Carme | - |
dc.contributor.author | Lorén Egea, José Gaspar | - |
dc.date.accessioned | 2020-07-22T08:09:22Z | - |
dc.date.available | 2020-07-22T08:09:22Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1466-5026 | - |
dc.identifier.uri | http://hdl.handle.net/2445/169268 | - |
dc.description.abstract | An analysis of the universal target (UT) sequence from the <i>cpn60</i> gene was performed in order to evaluate its usefulness in phylogenetic and taxonomic studies and as an identification marker for the genus <i>Aeromonas</i>. Sequences of 555 bp, corresponding to the UT region, were obtained from a collection of 35 strains representing all of the species and subspecies of <i>Aeromonas</i>. From the analysis of these sequences, a range of divergence of 0-23.3% was obtained, with a mean of 11.2±0.9 %. Comparative analyses between <i>cpn60</i> and <i>gyrB</i>, <i>rpoD</i> and 16S rRNA gene sequences were carried out from the same <i>Aeromonas</i> strain collection. Sequences of the <i>cpn60</i> UT region showed similar discriminatory power to <i>gyrB</i> and <i>rpoD</i> sequences. The phylogenetic relationships inferred from <i>cpn60</i> sequence distances indicated an excellent correlation with the present affiliation of <i>Aeromonas</i> species with the exception of <i>Aeromonas hydrophila</i> subsp. <i>dhakensis</i>, which appeared in a separate phylogenetic line, and <i>Aeromonas sharmana</i>, which exhibited a very loose phylogenetic relationship to the genus <i>Aeromonas</i>. Sequencing of <i>cpn60</i> from 33 additional <i>Aeromonas</i> strains also allowed us to establish intra- and interspecific threshold values. Intraspecific divergence rates were ≤3.5 %, while interspecific divergence rates fell between 3.7 and 16.9 %, excluding <i>A. sharmana</i>. In this study, <i>cpn60</i> UT sequencing was shown to be a universal, useful, simple and rapid method for the identification and phylogenetic affiliation of <i>Aeromonas</i> strains. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Microbiology Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1099/ijs.0.005413-0 | - |
dc.relation.ispartof | International Journal of Systematic and Evolutionary Microbiology, 2009, vol. 59, num. 8, p. 1976-1983 | - |
dc.relation.uri | https://doi.org/10.1099/ijs.0.005413-0 | - |
dc.rights | cc-by (c) Miñana i Galbis, David et al., 2009 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) | - |
dc.subject.classification | Bacteris | - |
dc.subject.classification | Filogènia | - |
dc.subject.classification | Genètica bacteriana | - |
dc.subject.classification | Microbiologia | - |
dc.subject.other | Bacteria | - |
dc.subject.other | Phylogeny | - |
dc.subject.other | Bacterial genetics | - |
dc.subject.other | Microbiology | - |
dc.title | Phylogenetic analysis and identification of Aeromonas species based on sequencing of the cpn60 universal target | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 576302 | - |
dc.date.updated | 2020-07-22T08:09:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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576302.pdf | 209.61 kB | Adobe PDF | View/Open |
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