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Title: Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration
Author: Garcia Puig, Anna
Mosquera Mayo, José Luís
Jimenez Delgao, Senda
Garcia Pastor, Cristina
Jorba, Ignasi
Navajas Navarro, Daniel
Canals, Francesc
Raya Chamorro, Ángel
Keywords: Malalties cardiovasculars
Models animals en la investigació
Biologia del desenvolupament
Cardiovascular diseases
Animal models in research
Developmental biology
Issue Date: Sep-2019
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. Here, we set out to characterize the ECM protein composition in adult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 days postamputation) time point analyzed. Regeneration associated with sharp increases in specific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopy that the changes in ECM composition translated to decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration.
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It is part of: Molecular & Cellular Proteomics, 2019, vol. 18, num. 9, p. 1745-1755
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ISSN: 1535-9476
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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