Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/171541
Title: | Whole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients |
Author: | Fernández Santiago, Rubén Merkel, Angelika Castellano, Giancarlo Heath, Simon Raya Chamorro, Ángel Tolosa, Eduardo Marti, Maria Jose Consiglio, Antonella Ezquerra, Mario |
Keywords: | Malaltia de Parkinson ADN Metilació Parkinson's disease DNA Methylation |
Issue Date: | 23-Jul-2019 |
Publisher: | BioMed Central |
Abstract: | Background: Parkinson's disease (PD) is characterized by the loss of midbrain dopaminergic neurons (DAn). Previously, we described the presence of DNA hyper- and hypo-methylation alterations in induced pluripotent stem cells (iPSC)-derived DAn from PD patients using the Illumina 450K array which prominently covers gene regulatory regions. Methods: To expand and contextualize previous findings, we performed the first whole-genome DNA bisulfite sequencing (WGBS) using iPSC-derived DAn from representative PD subjects: one sporadic PD (sPD) patient, one monogenic LRRK2-associated PD patient (L2PD), and one control. Results: At the whole-genome level, we detected global DNA hyper-methylation in the PD which was similarly spread across the genome in both sPD and L2PD and mostly affected intergenic regions. Conclusion: This study implements previous epigenetic knowledge in PD at a whole genome level providing the first comprehensive and unbiased CpG DNA methylation data using iPSC-derived DAn from PD patients. Our results indicate that DAn from monogenic or sporadic PD exhibit global DNA hyper-methylation changes. Findings from this exploratory study are to be validated in further studies analyzing other PD cell models and patient tissues. |
Note: | Reproducció del document publicat a: https://doi.org/10.1186/s13148-019-0701-6 |
It is part of: | Clinical Epigenetics, 2019, vol. 11 |
URI: | http://hdl.handle.net/2445/171541 |
Related resource: | https://doi.org/10.1186/s13148-019-0701-6 |
ISSN: | 1868-7075 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Patologia i Terapèutica Experimental) Publicacions de projectes de recerca finançats per la UE Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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