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http://hdl.handle.net/2445/171572
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DC Field | Value | Language |
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dc.contributor.author | Pais, Helio | - |
dc.contributor.author | Ruggero, Katia | - |
dc.contributor.author | Zhang, Jing | - |
dc.contributor.author | Al-Assar, Osama | - |
dc.contributor.author | Bery, Nicolas | - |
dc.contributor.author | Bhuller, Ravneet | - |
dc.contributor.author | Weston, Victoria | - |
dc.contributor.author | Kearns, Pamela R. | - |
dc.contributor.author | Mecucci, Cristina | - |
dc.contributor.author | Miller, Ami | - |
dc.contributor.author | Rabbitts, Terence H. | - |
dc.date.accessioned | 2020-10-28T08:19:35Z | - |
dc.date.available | 2020-10-28T08:19:35Z | - |
dc.date.issued | 2019-04-08 | - |
dc.identifier.uri | http://hdl.handle.net/2445/171572 | - |
dc.description.abstract | The surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Surfaceome DataBase Mining or Surfaceome DBM), to identify sets of upregulated cell surface protein mRNAs in an LMO2-mediated T-ALL mouse model and corroborated by protein detection using antibodies. In this model the leukemia initiating cells (LICs) comprise pre-leukaemic, differentiation inhibited thymocytes allowing us to provide a profile of the LIC surfaceome in which GPR56, CD53 and CD59a are co-expressed with CD25. Implementation of cell surface interaction assays demonstrates fluid interaction of surface proteins and CD25 is only internalized when co-localized with other proteins. The Surfaceome DBM approach to analyse cancer cell surfaceomes is a way to find targetable surface biomarkers for clinical conditions where RNA-seq data from normal and abnormal cell are available. | - |
dc.format.extent | 16 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-42214-w | - |
dc.relation.ispartof | Scientific Reports, 2019-04-08, Vol. 9, num.5760 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-019-42214-w | - |
dc.rights | cc by (c) Pais, Helio et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Marcadors bioquímics | - |
dc.subject.classification | Leucèmia | - |
dc.subject.mesh | Leukemia | - |
dc.subject.other | Biochemical markers | - |
dc.title | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2020-10-26T09:22:11Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30962539 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
This item is licensed under a Creative Commons License