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http://hdl.handle.net/2445/171756
Title: | Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates |
Author: | González Díaz, Aida Tubau, Fe Pinto, Miguel Sierra Urueña, Yanik Cubero, Meritxell Cámara, Jordi Ayats, Josefina Bajanca-Lavado, Paula Ardanuy Tisaire, María Carmen Marti, Sara |
Keywords: | Antígens bacterians Resistència als medicaments Polisacàrids Genomes Bacterial antigens Drug resistance Polysaccharides Genomes |
Issue Date: | 14-Mar-2019 |
Publisher: | Nature Publishing Group |
Abstract: | The human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A, N526K and V562I); quinolones, by alterations in GyrA (S84F and D88Y) and ParC (S84F and S138T); chloramphenicol, through the presence of catS; macrolides, via the presence of mel and mef(E)-carrying MEGA element; and tetracycline, through the presence of tet(M) and/or tet(B). Phylogenetic analysis revealed high genomic diversity when compared to the H. parainfluenzae genomes available on the NCBI, the isolates from this study being closely related to the Swiss XDR AE-2096513. A full capsular operon showing homology to that of H. influenzae was identified, in accordance with the observation of a capsular structure by TEM. This study describes for the first time a capsular operon in H. parainfluenzae, a major determinant of pathogenicity that may contribute to increased virulence in XDR clinical isolates. Moreover, phylogenetic analysis suggests the possible spread of an XDR-encapsulated strain in Europe. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-40812-2 |
It is part of: | Scientific Reports, 2019, vol. 9, num. 1, p. 4481 |
URI: | http://hdl.handle.net/2445/171756 |
Related resource: | https://doi.org/10.1038/s41598-019-40812-2 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Medicina) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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