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Title: Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
Author: González Díaz, Aida
Tubau, Fe
Pinto, Miguel
Sierra Urueña, Yanik
Cubero, Meritxell
Cámara, Jordi
Ayats, Josefina
Bajanca-Lavado, Paula
Ardanuy Tisaire, María Carmen
Marti, Sara
Keywords: Antígens bacterians
Resistència als medicaments
Bacterial antigens
Drug resistance
Issue Date: 14-Mar-2019
Publisher: Nature Publishing Group
Abstract: The human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A, N526K and V562I); quinolones, by alterations in GyrA (S84F and D88Y) and ParC (S84F and S138T); chloramphenicol, through the presence of catS; macrolides, via the presence of mel and mef(E)-carrying MEGA element; and tetracycline, through the presence of tet(M) and/or tet(B). Phylogenetic analysis revealed high genomic diversity when compared to the H. parainfluenzae genomes available on the NCBI, the isolates from this study being closely related to the Swiss XDR AE-2096513. A full capsular operon showing homology to that of H. influenzae was identified, in accordance with the observation of a capsular structure by TEM. This study describes for the first time a capsular operon in H. parainfluenzae, a major determinant of pathogenicity that may contribute to increased virulence in XDR clinical isolates. Moreover, phylogenetic analysis suggests the possible spread of an XDR-encapsulated strain in Europe.
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It is part of: Scientific Reports, 2019, vol. 9, num. 1, p. 4481
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Medicina)

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