Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/171945
Title: | Intestinal Inflammation Modulates the Epithelial Response to Butyrate in Patients With Inflammatory Bowel Disease |
Author: | Ferrer Picón, Elena Dotti, Isabella Corraliza Márquez, Ana Maria Mayorgas, Aida Esteller, Míriam Perales Losa, Carlos Ricart, Elena Masamunt, Maria Carme Carrasco García, Anna Tristán, Eva Esteve i Comas, Maria Salas Martínez, Azucena |
Keywords: | Malalties de l'aparell digestiu Colitis ulcerosa Malaltia de Crohn Digestive system diseases Ulcerative colitis Crohn's disease |
Issue Date: | Jan-2020 |
Publisher: | Wiley-Blackwell |
Abstract: | Background: Butyrate-producing gut bacteria are reduced in patients with active inflammatory bowel disease (IBD), supporting the hypothesis that butyrate supplementation may be beneficial in this setting. Nonetheless, earlier studies suggest that the oxidation of butyrate in IBD patients is altered. We propose that inflammation may decrease epithelial butyrate consumption. Methods: Non-IBD controls and IBD patients were recruited for the study. Stool samples were used for short-chain fatty acid and bacterial butyryl CoA:acetate CoA-transferase quantification. Colonic biopsies and ex vivo differentiated epithelial organoids (d-EpOCs) treated with bu- tyrate and/or tumor necrosis factor alpha (TNFα) were used for analyzing the expression of transporters MCT1 and ABCG2, metabolic enzyme ACADS, and butyrate receptor GPR43, and for butyrate metabolism and consumption assays. Results: We observed that lower stool content of butyrate-producing bacteria in active IBD patients did not correlate with decreased bu- tyrate concentrations. Indeed, the intestinal epithelial expression of MCT1, ABCG2, ACADS, and GPR43 was altered in active IBD patients. Nonetheless, d-EpOCs derived from IBD patients showed SLC16A1 (gene encoding for MCT1 protein), ABCG2, ACADS, and GPR43 expres- sion levels comparable to controls. Moreover, IBD- and non-IBD-derived d-EpOCs responded similarly to butyrate, as assessed by transcriptional regulation. TNFα significantly altered SLC16A1, ABCG2, and GPR43 transcription in d-EpOCs, mimicking the expression profile observed in biopsies from active IBD patients and resulting in reduced butyrate consumption. Conclusions: We provide evidence that the response to butyrate is not intrinsically altered in IBD patients. However, TNFα renders the epithe- lium less responsive to this metabolite, defeating the purpose of butyrate supplementation during active inflammation. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/ibd/izz119 |
It is part of: | Inflammatory Bowel Diseases, 2020, vol. 26, num. 1, p. 43-55 |
URI: | http://hdl.handle.net/2445/171945 |
Related resource: | https://doi.org/10.1093/ibd/izz119 |
ISSN: | 1078-0998 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
692112.pdf | 42.68 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License