Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/172416
Title: | Genomic characterization of individuals presenting extreme phenotypes of high and low risk to develop tobacco-induced lung cancer |
Author: | Fusco, Juan Pablo Pita, Guillermo Pajares, María José Andueza, Maria Pilar Patiño García, Ana Torres, Juan P. de Gurpide, Alfonso Zulueta, Javier Alonso, Rosario Alvarez, Nuria Pio, Ruben Melero, Ignacio Sanmamed, Miguel F. Rodriguez Ruiz, Maria Gil Bazo, Ignacio López Picazo, José María Casanova, Ciro Baz Davila, Rebeca Agudo, Antonio Lozano, Maria Dolores Gonzalez, Alvaro Sala Serra, Núria Ardanaz, Eva Benitez, Javier Montuenga, Luis M. González Neira, Anna Pérez Gracia, José Luis |
Keywords: | Càncer de pulmó Genòmica Lung cancer Genomics |
Issue Date: | 1-Jul-2018 |
Publisher: | Wiley |
Abstract: | Single nucleotide polymorphisms (SNPs) may modulate individual susceptibility to carcinogens. We designed a genome-wide association study to characterize individuals presenting extreme phenotypes of high and low risk to develop tobacco-induced non-small cell lung cancer (NSCLC), and we validated our results. We hypothesized that this strategy would enrich the frequencies of the alleles that contribute to the observed traits. We genotyped 2.37 million SNPs in 95 extreme phenotype individuals, that is: heavy smokers that either developed NSCLC at an early age (extreme cases); or did not present NSCLC at an advanced age (extreme controls), selected from a discovery set (n=3631). We validated significant SNPs in 133 additional subjects with extreme phenotypes selected from databases including >39,000 individuals. Two SNPs were validated: rs12660420 (p(combined)=5.66x10(-5); ORcombined=2.80), mapping to a noncoding transcript exon of PDE10A; and rs6835978 (p(combined)=1.02x10(-4); ORcombined=2.57), an intronic variant in ATP10D. We assessed the relevance of both proteins in early-stage NSCLC. PDE10A and ATP10D mRNA expressions correlated with survival in 821 stage I-II NSCLC patients (p=0.01 and p<0.0001). PDE10A protein expression correlated with survival in 149 patients with stage I-II NSCLC (p=0.002). In conclusion, we validated two variants associated with extreme phenotypes of high and low risk of developing tobacco-induced NSCLC. Our findings may allow to identify individuals presenting high and low risk to develop tobacco-induced NSCLC and to characterize molecular mechanisms of carcinogenesis and resistance to develop NSCLC. |
Note: | Reproducció del document publicat a: https://doi.org/10.1002/cam4.1500 |
It is part of: | Cancer Medicine, 2018, vol. 7, num. 7, p. 3474-3483 |
URI: | http://hdl.handle.net/2445/172416 |
Related resource: | https://doi.org/10.1002/cam4.1500 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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