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http://hdl.handle.net/2445/173049
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DC Field | Value | Language |
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dc.contributor.author | Grijalvo, Santiago | - |
dc.contributor.author | Clua, Anna | - |
dc.contributor.author | Eres, Marc | - |
dc.contributor.author | Gargallo Gómez, Raimundo | - |
dc.contributor.author | Eritja i Casadellà, Ramon | - |
dc.date.accessioned | 2021-01-11T11:17:33Z | - |
dc.date.available | 2021-01-11T11:17:33Z | - |
dc.date.issued | 2020-12-24 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/2445/173049 | - |
dc.description.abstract | Two G-quadruplex forming oligonucleotides [d(TG4T)4 and d(TG6T)4] were selected as two tetramolecular quadruplex nanostructures because of their demonstrated ability to be modified with hydrophobic molecules. This allowed us to synthesize two series of G-quadruplex conjugates that differed in the number of G-tetrads, as well as in the terminal position of the lipid modification. Both solution and solid-phase syntheses were carried out to yield the corresponding lipid oligonucleotide conjugates modified at their 3′- and 5′-termini, respectively. Biophysical studies confirmed that the presence of saturated alkyl chains with different lengths did not affect the G-quadruplex integrity, but increased the stability. Next, the G-quadruplex domain was added to an 18-mer antisense oligonucleotide. Gene silencing studies confirmed the ability of such G-rich oligonucleotides to facilitate the inhibition of target Renilla luciferase without showing signs of toxicity in tumor cell lines. | - |
dc.format.extent | 24 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms22010121 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2020, vol. 22, p. 121 | - |
dc.relation.uri | https://doi.org/10.3390/ijms22010121 | - |
dc.rights | cc-by (c) Grijalvo, Santiago et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Oligonucleòtids | - |
dc.subject.classification | Dicroisme circular | - |
dc.subject.classification | Lípids | - |
dc.subject.classification | Nanoestructures | - |
dc.subject.other | Oligonucleotides | - |
dc.subject.other | Circular dichroism | - |
dc.subject.other | Lipids | - |
dc.subject.other | Nanostructures | - |
dc.title | Tuning G-quadruplex nanostructures with lipids. Towards designing hybrid scaffolds for oligonucleotide delivery | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 705526 | - |
dc.date.updated | 2021-01-11T11:17:33Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33374392 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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File | Description | Size | Format | |
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705526.pdf | 2 MB | Adobe PDF | View/Open |
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