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DC Field | Value | Language |
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dc.contributor.author | Fernández Varo, Guillermo | - |
dc.contributor.author | Jiménez Povedano, Wladimiro | - |
dc.contributor.author | Marfà Bruix, Santiago | - |
dc.contributor.author | Morales Ruiz, Manuel | - |
dc.contributor.author | Oró Bozzini, Denise | - |
dc.contributor.author | Ribera Sabaté, Jordi | - |
dc.date.accessioned | 2021-01-19T17:27:18Z | - |
dc.date.available | 2021-01-19T17:27:18Z | - |
dc.date.issued | 2016-06-15 | - |
dc.identifier.uri | http://hdl.handle.net/2445/173186 | - |
dc.description.abstract | At present, several procedures are used for staging liver fibrosis. However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease. Thus, this study was designed to unveil new non-invasive biomarkers of liver fibrosis in an in vivo model of fibrosis/cirrhosis induction by CCl4 inhalation by using a label-free quantitative LC-MS/MS approach. We analyzed 94 serum samples from adult Wistar rats with different degrees of liver fibrosis and 36 control rats. Firstly, serum samples from 18 CCl4-treated rats were clustered into three different groups according to the severity of hepatic and the serum proteome was characterized by label-free LC-MS/MS. Furthermore, three different pooled serum samples obtained from 16 control Wistar rats were also analyzed. Based on the proteomic data obtained, we performed a multivariate analysis which displayed three main cell signaling pathways altered in fibrosis. In cirrhosis, more biological imbalances were detected as well as multi-organ alterations. In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins. The results were validated by ELISA in an independent group of 76 fibrotic/cirrhotic rats and 20 controls which confirmed SIPA1L1 as a potential non-invasive biomarker of liver fibrosis. In particular, SIPA1L1 showed a clear diminution in serum samples from fibrotic/cirrhotic rats and a great accuracy at identifying early fibrotic stages. In conclusion, the proteomic analysis of serum samples from CCl4-treated rats has enabled the identification of SIPA1L1 as a non-invasive marker of early liver fibrosis. © 2016. Published by The Company of Biologists Ltd. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1242/bio.018887 | - |
dc.relation.ispartof | Biology Open, 2016, vol. 5, p. 858-865 | - |
dc.relation.uri | https://doi.org/10.1242/bio.018887 | - |
dc.rights | cc by (c) Fernández Varo et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Marcadors bioquímics | - |
dc.subject.classification | Proteòmica | - |
dc.subject.classification | Malalties del fetge | - |
dc.subject.other | Biochemical markers | - |
dc.subject.other | Proteomics | - |
dc.subject.other | Liver diseases | - |
dc.title | Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats | - |
dc.type | info:eu-repo/semantics/article | - |
dc.date.updated | 2021-01-15T09:35:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 2106119 | - |
dc.identifier.pmid | 27230648 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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