Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/173364
Title: | Photoswitchable dynasore analogs to control endocytosis with light |
Author: | Camarero, Núria Trapero, Ana Pérez Jiménez, Ariadna Macia, Eric Gomila Juaneda, Alexandre Gorostiza Langa, Pablo Ignacio Nevola, Laura Llobet Berenguer, Artur, 1972- Llebaria, Amadeu Hernando, Jordi Giralt Lledó, Ernest |
Keywords: | Interacció cel·lular Espectroscòpia molecular Cell interaction Molecular spectroscopy |
Issue Date: | 2020 |
Publisher: | Royal Society of Chemistry |
Abstract: | The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single- wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1039/d0sc03820b |
It is part of: | Chemical Science, 2020, vol. 11, p. 8981-8988 |
URI: | http://hdl.handle.net/2445/173364 |
Related resource: | https://doi.org/10.1039/d0sc03820b |
ISSN: | 2041-6520 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) Articles publicats en revistes (Química Inorgànica i Orgànica) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
703957.pdf | 758.67 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.