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http://hdl.handle.net/2445/173367
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DC Field | Value | Language |
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dc.contributor.author | Matteini, Francesca | - |
dc.contributor.author | Andresini, Oriella | - |
dc.contributor.author | Petrai, Stefano | - |
dc.contributor.author | Battistelli, Cecilia | - |
dc.contributor.author | Rossi, Marianna Nicoletta | - |
dc.contributor.author | Maione, Rossella | - |
dc.date.accessioned | 2021-01-25T10:37:30Z | - |
dc.date.available | 2021-01-25T10:37:30Z | - |
dc.date.issued | 2020-09-15 | - |
dc.identifier.uri | http://hdl.handle.net/2445/173367 | - |
dc.description.abstract | The myogenic factor MyoD regulates skeletal muscle differentiation by interacting with a variety of chromatin-modifying complexes. Although MyoD can induce and maintain chromatin accessibility at its target genes, its binding and trans-activation ability can be limited by some types of not fully characterized epigenetic constraints. In this work we analysed the role of PARP1 in regulating MyoD-dependent gene expression. PARP1 is a chromatin-associated enzyme, playing a well recognized role in DNA repair and that is implicated in transcriptional regulation. PARP1 affects gene expression through multiple mechanisms, often involving the Poly(ADP-ribosyl)ation of chromatin proteins. In line with PARP1 down-regulation during differentiation, we observed that PARP1 depletion boosts the up-regulation of MyoD targets, such as p57, myogenin, Mef2C and p21, while its re-expression reverts this effect. We also found that PARP1 interacts with some MyoD-binding regions and that its presence, independently of the enzymatic activity, interferes with MyoD recruitment and gene induction. We finally suggest a relationship between the binding of PARP1 and the loss of the activating histone modification H3K4me3 at MyoD-binding regions. This work highlights not only a novel player in the epigenetic control of myogenesis, but also a repressive and catalytic-independent mechanisms by which PARP1 regulates transcription. | - |
dc.format.extent | 18 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Research | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-72155-8 | - |
dc.relation.ispartof | Scientific Reports, 2020, vol. 10 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-020-72155-8 | - |
dc.rights | cc by (c) Matteini et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Múscul estriat | - |
dc.subject.classification | Miogènesi | - |
dc.subject.other | Striated muscle | - |
dc.subject.other | Myogenesis | - |
dc.title | Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2021-01-25T08:05:01Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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MatteiniF.pdf | 3.28 MB | Adobe PDF | View/Open |
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