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Title: Genomic profiling of primary and recurrent Adult Granulosa Cell Tumors of the Ovary
Author: Cruz Paula, Arnaud Da
Silva, Edaise M. da
Segura, Sheila E.
Pareja, Fresia
Bi, Rui
Selenica, Pier
Kim, Sarah H.
Ferrando, Lorenzo
Vahdatinia, Mahsa
Soslow, Robert A.
Vidal-Bel, August
Gatius, Sonia
Przybycin, Christopher G.
Abu-Rustum, Nadeem R.
Matias-Guiu, Xavier
Rubin, Brian P.
Reis-Filho, Jorge S.
DeLair, Deborah F.
Weigelt, Britta
Keywords: Càncer d'ovari
Cèl·lules canceroses
Ovarian cancer
Cancer cells
Issue Date: 12-Mar-2020
Publisher: Nature Publishing Group
Abstract: Adult-type granulosa cell tumor (aGCT) is a rare malignant ovarian sex cord-stromal tumor, harboring recurrent FOXL2 c.C402G/p.C134W hotspot mutations in 97% of cases. These tumors are considered to have a favorable prognosis, however aGCTs have a tendency for local spread and late recurrences, which are associated with poor survival rates. We sought to determine the genetic alterations associated with aGCT disease progression. We subjected primary non-recurrent aGCTs (n = 7), primary aGCTs that subsequently recurred (n = 9) and their matched recurrences (n = 9), and aGCT recurrences without matched primary tumors (n = 10) to targeted massively parallel sequencing of ≥410 cancer-related genes. In addition, three primary non-recurrent aGCTs and nine aGCT recurrences were subjected to FOXL2 and TERT promoter Sanger sequencing analysis. All aGCTs harbored the FOXL2 C134W hotspot mutation. TERT promoter mutations were found to be significantly more frequent in recurrent (18/28, 64%) than primary aGCTs (5/19, 26%, p = 0.017). In addition, mutations affecting TP53, MED12, and TET2 were restricted to aGCT recurrences. Pathway annotation of altered genes demonstrated that aGCT recurrences displayed an enrichment for genetic alterations affecting cell cycle pathway-related genes. Analysis of paired primary and recurrent aGCTs revealed that TERT promoter mutations were either present in both primary tumors and matched recurrences or were restricted to the recurrence and absent in the respective primary aGCT. Clonal composition analysis of these paired samples further revealed that aGCTs display intra-tumor genetic heterogeneity and harbor multiple clones at diagnosis and relapse. We observed that in a subset of cases, recurrences acquired additional genetic alterations not present in primary aGCTs, including TERT, MED12, and TP53 mutations and CDKN2A/B homozygous deletions. Albeit harboring relatively simple genomes, our data provide evidence to suggest that aGCTs are genetically heterogeneous tumors and that TERT promoter mutations and/or genetic alterations affecting other cell cycle-related genes may be associated with disease progression and recurrences.
Note: Versió postprint del document publicat a:
It is part of: Modern Pathology, 2020, vol. 33, p. 1606-1617
Related resource:
ISSN: 0893-3952
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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