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Title: A Two-Photon Probe Based on Naphthalimide-Styrene Fluorophore for the In Vivo Tracking of Cellular Senescence
Author: Lozano Torres, Beatriz
Blandez, Juan F.
Galiana, Irene
López Domínguez, José A.
Rovira, Miguel
Paez-Ribes, Marta
González Gualda, Estela
Muñoz Espín, Daniel
Serrano Marugán, Manuel
Sancenón, Félix
Martínez Máñez, Ramón
Keywords: Regeneració (Biologia)
Regeneration (Biology)
Issue Date: 27-Jan-2021
Publisher: American Chemical Society
Abstract: Cellular senescence is a state of stable cell cycle arrest that can negatively affect the regenerative capacities of tissues and can contribute to inflammation and the progression of various aging-related diseases. Advances in the in vivo detection of cellular senescence are still crucial to monitor the action of senolytic drugs and to assess the early onset or accumulation of senescent cells. Here, we describe a naphthalimide-styrene-based probe (HeckGal) for the detection of cellular senescence both in vitro and in vivo. HeckGal is hydrolyzed by the increased lysosomal β-galactosidase activity of senescent cells, resulting in fluorescence emission. The probe was validated in vitro using normal human fibroblasts and various cancer cell lines undergoing senescence induced by different stress stimuli. Remarkably, HeckGal was also validated in vivo in an orthotopic breast cancer mouse model treated with senescence-inducing chemotherapy and in a renal fibrosis mouse model. In all cases, HeckGal allowed the unambiguous detection of senescence in vitro as well as in tissues and tumors in vivo. This work is expected to provide a potential technology for senescence detection in aged or damaged tissues.
Note: Versió postprint del document publicat a:
It is part of: Analytical Chemistry, 2021
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Appears in Collections:Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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