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http://hdl.handle.net/2445/174027
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DC Field | Value | Language |
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dc.contributor.author | Mertens, Christina | - |
dc.contributor.author | Kuchler, Laura | - |
dc.contributor.author | Sola Martínez, Anna | - |
dc.contributor.author | Guiteras, Roser | - |
dc.contributor.author | Grein, Stephan | - |
dc.contributor.author | Brüne, Bernhard, 1957- | - |
dc.contributor.author | von Knethen, Andreas | - |
dc.contributor.author | Jung, Michaela | - |
dc.date.accessioned | 2021-02-18T18:35:47Z | - |
dc.date.available | 2021-02-18T18:35:47Z | - |
dc.date.issued | 2020-10-01 | - |
dc.identifier.other | Macrophages | - |
dc.identifier.uri | http://hdl.handle.net/2445/174027 | - |
dc.description.abstract | During the course of sepsis in critically ill patients, kidney dysfunction and damage are among the first events of a complex scenario toward multi-organ failure and patient death. Acute kidney injury triggers the release of lipocalin-2 (Lcn-2), which is involved in both renal injury and recovery. Taking into account that Lcn-2 binds and transports iron with high affinity, we aimed at clarifying if Lcn-2 fulfills different biological functions according to its iron-loading status and its cellular source during sepsis-induced kidney failure. We assessed Lcn-2 levels both in serum and in the supernatant of short-term cultured renal macrophages (M phi) as well as renal tubular epithelial cells (TEC) isolated from either Sham-operated or cecal ligation and puncture (CLP)-treated septic mice. Total kidney iron content was analyzed by Perls' staining, while Lcn-2-bound iron in the supernatants of short-term cultured cells was determined by atomic absorption spectroscopy. Lcn-2 protein in serum was rapidly up-regulated at 6 h after sepsis induction and subsequently increased up to 48 h. Lcn-2-levels in the supernatant of TEC peaked at 24 h and were low at 48 h with no change in its iron-loading. In contrast, in renal M phi Lcn-2 was low at 24 h, but increased at 48 h, where it mainly appeared in its iron-bound form. Whereas TEC-secreted, iron-free Lcn-2 was associated with renal injury, increased M phi-released iron-bound Lcn-2 was linked to renal recovery. Therefore, we hypothesized that both the cellular source of Lcn-2 as well as its iron-load crucially adds to its biological function during sepsis-induced renal injury. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms21207527 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2020, vol. 21, num. 20 | - |
dc.relation.uri | https://doi.org/10.3390/ijms21207527 | - |
dc.rights | cc by (c) Mertens et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Malalties del ronyó | - |
dc.subject.classification | Macròfags | - |
dc.subject.classification | Cèl·lules epitelials | - |
dc.subject.other | Kidney diseases | - |
dc.subject.other | Epithelial cells | - |
dc.title | Macrophage-Derived Iron-Bound Lipocalin-2 Correlates with Renal Recovery Markers Following Sepsis-Induced Kidney Damage | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2021-02-08T10:31:41Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33065981 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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MertensC.pdf | 2.66 MB | Adobe PDF | View/Open |
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