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Title: | G-quadruplex binding properties of a potent PARP-1 inhibitor derived from 7-azaindole-1-carboxamide |
Author: | Dallavalle, Sabrina Musso, Loana Artali, Roberto Aviñó Andrés, Anna Scaglioni, Leonardo Eritja i Casadellà, Ramon Gargallo Gómez, Raimundo Mazzini, Stefania |
Keywords: | G-estructures Disseny de medicaments Càncer G-structures Drug design Cancer |
Issue Date: | 16-Feb-2021 |
Publisher: | Nature Publishing Group |
Abstract: | oly ADP-ribose polymerases (PARP) are key proteins involved in DNA repair, maintenance as well as regulation of programmed cell death. For this reason they are important therapeutic targets for cancer treatment. Recent studies have revealed a close interplay between PARP1 recruitment and G-quadruplex stabilization, showing that PARP enzymes are activated upon treatment with a G4 ligand. In this work the DNA binding properties of a PARP-1 inhibitor derived from 7-azaindole-1-carboxamide, (2-[6-(4-pyrrolidin-1-ylmethyl-phenyl)-pyrrolo[2,3-b]pyridin-1-yl]-acetamide, compound 1) with model duplex and quadruplex DNA oligomers were studied by NMR, CD, fluorescence and molecular modelling. We provide evidence that compound 1 is a strong G-quadruplex binder. In addition we provide molecular details of the interaction of compound 1 with two model G-quadruplex structures: the single repeat of human telomeres, d(TTAGGGT)4, and the c-MYC promoter Pu22 sequence. The formation of defined and strong complexes with G-quadruplex models suggests a dual G4 stabilization/PARP inhibition mechanism of action for compound 1 and provides the molecular bases of its therapeutic potential. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-021-83474-9 |
It is part of: | Scientific Reports, 2021, vol. 11, num. 3869 |
URI: | http://hdl.handle.net/2445/174097 |
Related resource: | https://doi.org/10.1038/s41598-021-83474-9 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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