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Title: | Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray |
Author: | Hernández Altarmirano, J. Mabel Fernández Ugidos, Irene Palazón Barandela, Javier Bonfill Baldrich, Ma. Mercedes García Angulo, Penélope Álvarez, Jesús Acebes, José L. Bye, Robert Encina, Antonio |
Keywords: | Plantes medicinals Metabòlits Metabolisme cel·lular Medicinal plants Metabolites Cell metabolism |
Issue Date: | 15-Jul-2020 |
Publisher: | MDPI |
Abstract: | Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production. Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions). In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4. After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 g/g) than the sonication assay (120 g/g), both giving far higher yields than the prick assay (19 g/g). Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR). Hairy roots from cocultivation (six months-old) accumulated as much as 1086 g/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase. This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/molecules25143231 |
It is part of: | Molecules, 2020, vol. 25(14), num. 3231 |
URI: | https://hdl.handle.net/2445/174302 |
Related resource: | https://doi.org/10.3390/molecules25143231 |
ISSN: | 1420-3049 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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