Please use this identifier to cite or link to this item:
Title: Functional consequences of the variable stoichiometry of the Kv1.3-KCNE4 complex
Author: Solé i Codina, Laura
Sastre Martinez, Daniel
Colomer-Molera, Magalí
Vallejo Gracia, Albert
Roig Merino, Sara Raquel
Perez-Verdaguer, Mireia
Lillo, Pilar
Tamkun, Michael M.
Felipe Campo, Antonio
Keywords: Canals de potassi
Sistema immunitari
Potassium channels
Immune system
Issue Date: 2-May-2020
Publisher: MDPI
Abstract: The voltage-gated potassium channel Kv1.3 plays a crucial role during the immune response. The channel forms oligomeric complexes by associating with several modulatory subunits. KCNE4, one of the five members of the KCNE family, binds to Kv1.3, altering channel activity and membrane expression. The association of KCNEs with Kv channels is the subject of numerous studies, and the stoichiometry of such associations has led to an ongoing debate. The number of KCNE4 subunits that can interact and modulate Kv1.3 is unknown. KCNE4 transfers important elements to the Kv1.3 channelosome that negatively regulate channel function, thereby fine-tuning leukocyte physiology. The aim of this study was to determine the stoichiometry of the functional Kv1.3-KCNE4 complex. We demonstrate that as many as four KCNE4 subunits can bind to the same Kv1.3 channel, indicating a variable Kv1.3-KCNE4 stoichiometry. While increasing the number of KCNE4 subunits steadily slowed the activation of the channel and decreased the abundance of Kv1.3 at the cell surface, the presence of a single KCNE4 peptide was suffcient for the cooperative enhancement of the inactivating function of the channel. This variable architecture, which depends on KCNE4 availability, differentially affects Kv1.3 function. Therefore, our data indicate that the physiological remodeling of KCNE4 triggers functional consequences for Kv1.3, thus affecting cell physiology.
Note: Reproducció del document publicat a:
It is part of: Cells, 2020, vol. 9(5), num. 1128
Related resource:
ISSN: 2073-4409
Appears in Collections:Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Files in This Item:
File Description SizeFormat 
703312.pdf4.9 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons