Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/174577
Title: | Apoptosis, G1 Phase Stall, and Premature Differentiation Account for Low Chimeric Competence of Human and Rhesus Monkey Naive Pluripotent Stem Cells |
Author: | Aksoy, Irène Afanassieff, Marielle Bellemin Ménard, Angèle Bourillot, Pierre Yves Cortay, Véronique Dehay, Colette Dirheimer, Manon Doerflinger, Nathalie Joly, Thierry Lynch, Cian Marcy, Guillaume Masfaraud, Etienne Mayère, Chloé Moulin, Anaïs Raineteau, Olivier Rognard, Cloé Savatier, Pierre Serrano Marugán, Manuel Wianny, Florence |
Keywords: | Cèl·lules mare Mones Home Stem cells Monkeys Human |
Issue Date: | 12-Jan-2021 |
Publisher: | Cell Press |
Abstract: | After reprogramming to naive pluripotency, human pluripotent stem cells (PSCs) still exhibit very low ability to make interspecies chimeras. Whether this is because they are inherently devoid of the attributes of chimeric competency or because naive PSCs cannot colonize embryos from distant species remains to be elucidated. Here, we have used different types of mouse, human, and rhesus monkey naive PSCs and analyzed their ability to colonize rabbit and cynomolgus monkey embryos. Mouse embryonic stem cells (ESCs) remained mitotically active and efficiently colonized host embryos. In contrast, primate naive PSCs colonized host embryos with much lower efficiency. Unlike mouse ESCs, they slowed DNA replication after dissociation and, after injection into host embryos, they stalled in the G1 phase and differentiated prematurely, regardless of host species. We conclude that human and non-human primate naive PSCs do not efficiently make chimeras because they are inherently unfit to remain mitotically active during colonization. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2020.12.004 |
It is part of: | Stem Cell Reports, 2021, Vol.16, num.1, p. 56-74 |
URI: | http://hdl.handle.net/2445/174577 |
Related resource: | https://doi.org/10.1016/j.stemcr.2020.12.004 |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
12541_6467667_savatier_stemcellrep_2021.pdf | 8.31 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License