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Title: Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment
Author: Urdinguio, Rocío G.
Lopez, Virginia
Bayón, Gustavo F.
Diaz de la guardia, Rafael
Sierra, Marta I.
García Toraño, Estela
Perez, Raúl F.
García, María G.
Carella, Antonella
Pruneda, Patricia C.
Prieto, Cristina
Dmitrijeva, Marija
Santamarina, Pablo
Belmonte, Thalía
Mangas, Cristina
Diaconu, Elena
Ferrero, Cecilia
Tejedor, Juan Ramón
Fernandez Morera, Juan Luis
Bravo, Cristina
Bueno, Clara
Sanjuan Pla, Alejandra
Rodriguez, Ramon M.
Suarez Alvarez, Beatriz
López Larrea, Carlos
Bernal, Teresa
Colado, Enrique
Balbín, Milagros
García Suarez, Olivia
Chiara, María Dolores
Sáenz de Santa María, Inés
Rodríguez, Francisco
Pando Sandoval, Ana
Rodrigo, Luis
Santos, Laura
Salas, Anna
Vallejo Díaz, Jesús
Carrera, Ana C.
Rico, Daniel
Hernández López, Inmaculada
Vayá, Amparo
Ricart, Josep M.
Seto, Edward
Sima Teruel, Núria
Vaquero García, Alejandro
Valledor, Luis
Cañal, Maria Jesus
Pisano, David
Graña Castro, Osvaldo
Thomas, Tim
Voss, Anne K.
Menéndez, Pablo
Villar Garea, Ana
Deutzmann, Rainer
Fernandez, Agustín F.
Fraga, Mario F.
Keywords: Cromatina
Mort cel·lular
Cell death
Issue Date: 29-Mar-2019
Publisher: Oxford University Press
Abstract: Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.
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It is part of: Nucleic Acids Research, 2019, vol. 47, num. 10, p. 5016-5037
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Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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