Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/174905
Title: | Probing the Kinetic and Thermodynamic Fingerprints of Anti-EGF Nanobodies by Surface Plasmon Resonance |
Author: | Guardiola Bagán, Salvador Varese, Monica Taulés i Marín, Marta Díaz Lobo, Mireia García Arroyo, Jesús Giralt Lledó, Ernest |
Keywords: | Antígens tumorals Biofísica Reconeixement molecular Tumor antigens Biophysics Molecular recognition |
Issue Date: | 26-Jun-2020 |
Publisher: | MDPI |
Abstract: | Despite the widespread use of antibodies in clinical applications, the precise molecular mechanisms underlying antibody-antigen (Ab-Ag) interactions are often poorly understood. In this study, we exploit the technical features of a typical surface plasmon resonance (SPR) biosensor to dissect the kinetic and thermodynamic components that govern the binding of single-domain Ab or nanobodies to their target antigen, epidermal growth factor (EGF), a key oncogenic protein that is involved in tumour progression. By carefully tuning the experimental conditions and transforming the kinetic data into equilibrium constants, we reveal the complete picture of binding thermodynamics, including the energetics of the complex-formation transition state. This approach, performed using an experimentally simple and high-throughput setup, is expected to facilitate mechanistic studies of Ab-based therapies and, importantly, promote the rational development of new biological drugs with suitable properties. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ph13060134 |
It is part of: | Pharmaceuticals, 2020, vol. 13(6), num. 134 |
URI: | https://hdl.handle.net/2445/174905 |
Related resource: | https://doi.org/10.3390/ph13060134 |
ISSN: | 1424-8247 |
Appears in Collections: | Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB)) Articles publicats en revistes (Química Inorgànica i Orgànica) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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