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Title: The Janus Role of Adhesion in Chondrogenesis
Author: Casanellas, Ignasi
Lagunas, Anna
Vida, Yolanda
Pérez Inestrosa, Ezequiel
Andrades, José A.
Becerra, José
Samitier i Martí, Josep
Keywords: Condrogènesi
Malalties dels cartílags
Cartilage diseases
Issue Date: 24-Jul-2020
Publisher: MDPI
Abstract: Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
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It is part of: International Journal of Molecular Sciences, 2020, vol. 21(15), num. 5269
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ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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