Please use this identifier to cite or link to this item:
Title: Exploring macrophage cell therapy on diabetic kidney disease
Author: Guiteras, Roser
Sola Martínez, Anna
Flaquer, Maria
Manonelles, Anna
Hotter, Georgina
Cruzado, Josep Ma.
Keywords: Macròfags
Insuficiència renal crònica
Nefropaties diabètiques
Chronic renal failure
Diabetic nephropathies
Issue Date: 2019
Publisher: John Wiley & Sons
Abstract: Alternatively activated macrophages (M2) have regenerative properties and shown promise as cell therapy in chronic kidney disease. However, M2 plasticity is one of the major hurdles to overcome. Our previous studies showed that genetically modified macrophages stabilized by neutrophil gelatinase‐associated lipocalin (NGAL) were able to preserve their M2 phenotype. Nowadays, little is known about M2 macrophage effects in diabetic kidney disease (DKD). The aim of the study was to investigate the therapeutic effect of both bone marrow‐derived M2 (BM‐фM2) and ф‐NGAL macrophages in the db/db mice. Seventeen‐week‐old mice with established DKD were divided into five treatment groups with their controls: D+BM‐фM2; D+ф‐BM; D+ф‐NGAL; D+ф‐RAW; D+SHAM and non‐diabetic (ND) (db/‐ and C57bl/6J) animals. We infused 1 × 106 macrophages twice, at baseline and 2 weeks thereafter. BM‐фM2 did not show any therapeutic effect whereas ф‐ NGAL significantly reduced albuminuria and renal fibrosis. The ф‐NGAL therapy increased the anti‐inflammatory IL‐10 and reduced some pro‐inflammatory cytoki nes, reduced the proportion of M1 glomerular macrophages and podocyte loss and was associated with a significant decrease of renal TGF‐β1. Overall, our study provides evidence that ф‐NGAL macrophage cell therapy has a therapeutic effect on DKD probably by modulation of the renal inflammatory response caused by the diabetic milieu.
Note: Reproducció del document publicat a:
It is part of: Journal of Cellular and Molecular Medicine, 2019, vol. 23, num. 2, p. 841-851
Related resource:
ISSN: 1582-1838
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Files in This Item:
File Description SizeFormat 
700031.pdf2.86 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons