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|Title:||Effect of cocoa-enriched diets on lymphocytes involved in adjuvant arthritis in rats|
|Author:||Ramos Romero, Sara|
Pérez-Cano, Francisco J.
Castellote i Bargalló, M. Cristina
Franch i Masferrer, Àngels
|Publisher:||Cambridge University Press|
|Abstract:||Cocoa and its flavonoids have potential anti-inflammatory properties in vitro and in acute inflammation models in vivo. The aim of the present study was to ascertain the effects of two cocoa-enriched diets on adjuvant arthritis (AA) in rats, considering not only clinical and biochemical inflammatory indices, but also antibody response and lymphocyte composition. Female Wistar rats were fed with a 5 or 10 % cocoa-enriched diet beginning 2 weeks before arthritis induction and until the end of the study. AA was induced by an intradermal injection of heat-killed Mycobacterium butyricum suspension. The hind-paw swelling (plethysmometry), serum anti-mycobacterial antibody concentration (ELISA), blood and inguinal lymph node lymphocyte subset percentage (flow cytometry), and IL-2, interferon γ and PGE2 released from splenocytes (ELISA) were assessed. Although the cocoa diets had no significant effect on hind-paw swelling, a tendency to reduce it was observed at the end of the study. Cocoa-enriched diets were able to decrease the serum anti-mycobacterial antibody concentration and the splenocyte PGE2 production, as well as the proportion of T-helper (Th) lymphocytes in blood and regional lymph nodes, which probably includes cells responsible for the arthritic process. The cocoa diets prevented a decrease in the proportion of regulatory T-cells in blood and a disequilibrium between inguinal lymph node natural killer (NK) CD8+ and NK CD8− subsets. In conclusion, the cocoa-enriched diets during AA were not able to significantly decrease joint inflammation but modified Th-cell proportions and prevented specific antibody synthesis.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1017/S0007114511003035|
|It is part of:||British Journal of Nutrition, 2012, vol. 107, num. 3, p. 378-387|
|Appears in Collections:||Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)|
Articles publicats en revistes (Bioquímica i Fisiologia)
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