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http://hdl.handle.net/2445/175277
Title: | Effect of cocoa-enriched diets on lymphocytes involved in adjuvant arthritis in rats |
Author: | Ramos Romero, Sara Pérez-Cano, Francisco J. Castellote i Bargalló, M. Cristina Castell, Margarida Franch i Masferrer, Àngels |
Keywords: | Cacau Limfòcits Artritis Cocoa Lymphocytes Arthritis |
Issue Date: | 2012 |
Publisher: | Cambridge University Press |
Abstract: | Cocoa and its flavonoids have potential anti-inflammatory properties in vitro and in acute inflammation models in vivo. The aim of the present study was to ascertain the effects of two cocoa-enriched diets on adjuvant arthritis (AA) in rats, considering not only clinical and biochemical inflammatory indices, but also antibody response and lymphocyte composition. Female Wistar rats were fed with a 5 or 10 % cocoa-enriched diet beginning 2 weeks before arthritis induction and until the end of the study. AA was induced by an intradermal injection of heat-killed Mycobacterium butyricum suspension. The hind-paw swelling (plethysmometry), serum anti-mycobacterial antibody concentration (ELISA), blood and inguinal lymph node lymphocyte subset percentage (flow cytometry), and IL-2, interferon γ and PGE2 released from splenocytes (ELISA) were assessed. Although the cocoa diets had no significant effect on hind-paw swelling, a tendency to reduce it was observed at the end of the study. Cocoa-enriched diets were able to decrease the serum anti-mycobacterial antibody concentration and the splenocyte PGE2 production, as well as the proportion of T-helper (Th) lymphocytes in blood and regional lymph nodes, which probably includes cells responsible for the arthritic process. The cocoa diets prevented a decrease in the proportion of regulatory T-cells in blood and a disequilibrium between inguinal lymph node natural killer (NK) CD8+ and NK CD8− subsets. In conclusion, the cocoa-enriched diets during AA were not able to significantly decrease joint inflammation but modified Th-cell proportions and prevented specific antibody synthesis. |
Note: | Reproducció del document publicat a: https://doi.org/10.1017/S0007114511003035 |
It is part of: | British Journal of Nutrition, 2012, vol. 107, num. 3, p. 378-387 |
URI: | http://hdl.handle.net/2445/175277 |
Related resource: | https://doi.org/10.1017/S0007114511003035 |
ISSN: | 0007-1145 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) Articles publicats en revistes (Bioquímica i Fisiologia) |
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