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Title: | Strong-LAMP Assay Based on a Strongyloides spp.-Derived Partial Sequence in the 18S rRNA as Potential Biomarker for Strongyloidiasis Diagnosis in Human Urine Samples |
Author: | Fernández Soto, Pedro Celis Giraldo, Carmen T. Collar-Fernández, Coralina Gorgojo, Óscar Camargo, Milena Muñoz Gutiérrez, José Salas Coronas, Joaquín Patarroyo, Manuel A. Muro, Antonio |
Keywords: | Malalties parasitàries Helmints Marcadors bioquímics Anàlisi d'orina Parasitic diseases Helminths Biochemical markers Urine analysis |
Issue Date: | 31-May-2020 |
Publisher: | IOS Press |
Abstract: | Human strongyloidiasis a soil-transmitted infection caused by Strongyloides stercoralis is one of the most neglected amongst the so-called Neglected Tropical Diseases (NTDs). S. stercoralis is a nematode, which is distributed worldwide; it has been estimated that it could affect millions of people, mainly in tropical and subtropical endemic regions. The difficulties of diagnosis lead to infection rates being underreported. Asymptomatic patients have chronic infections that can lead to severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. Strongyloidiasis can easily be misdiagnosed because conventional faecal-based techniques lack of sensitivity for the morphological identification of infective larvae in faeces. None of the currently used molecular methods have used urine samples as an alternative to faecal samples for diagnosing strongyloidiasis. This study was thus aimed at comparing, for the first time, the use of a new loop-mediated isothermal amplification (LAMP) molecular assay (Strong-LAMP) to traditional methods on patients' urine samples. Twenty-four urine samples were taken from patients included in a study involving two Spanish hospitals for strongyloidiasis screening using parasitological and serological tests. Strongyloides larvae were found in 11 patients' faecal samples, thereby ascertaining that they had the disease. Other patients had high antibody titres but no larvae were found in their faeces. All urine samples were analysed by PCR and Strong-LAMP assay. No amplification occurred when using PCR. Strong-LAMP led to detecting S. stercoralis DNA in urine samples from patients having previously confirmed strongyloidiasis by parasitological tests and/or a suspicion of being infected by serological ones. The Strong-LAMP assay is a useful molecular tool for research regarding strongyloidiasis in human urine samples. After further validation, the Strong-LAMP assay could also be used for complementary and effective diagnosis of strongyloidiasis in a clinical setting. |
Note: | Reproducció del document publicat a: https://doi.org/10.1155/2020/5265198 |
It is part of: | Disease Markers, 2020, p. 5265198 |
URI: | http://hdl.handle.net/2445/175509 |
Related resource: | https://doi.org/10.1155/2020/5265198 |
ISSN: | 0278-0240 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Medicina) |
Files in This Item:
File | Description | Size | Format | |
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702796.pdf | 1.05 MB | Adobe PDF | View/Open |
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