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DC Field | Value | Language |
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dc.contributor.author | Bisoffi, Zeno | - |
dc.contributor.author | Buonfrate, Dora | - |
dc.contributor.author | Sequi, Marco | - |
dc.contributor.author | Mejia, Rojelio | - |
dc.contributor.author | Cimino, Rubén O. | - |
dc.contributor.author | Krolewiecki, Alejandro J. | - |
dc.contributor.author | Albonico, Marco | - |
dc.contributor.author | Gobbo, Maria | - |
dc.contributor.author | Bonafini, Stefania | - |
dc.contributor.author | Angheben, Andrea | - |
dc.contributor.author | Requena-Méndez, Ana | - |
dc.contributor.author | Muñoz Gutiérrez, José | - |
dc.contributor.author | Nutman, Thomas B. | - |
dc.date.accessioned | 2021-03-22T14:19:50Z | - |
dc.date.available | 2021-03-22T14:19:50Z | - |
dc.date.issued | 2014-01-09 | - |
dc.identifier.issn | 1935-2735 | - |
dc.identifier.uri | http://hdl.handle.net/2445/175538 | - |
dc.description.abstract | Background: The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ('gold') standard. Methods: The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests). Results: According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity. Conclusions: NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0002640 | - |
dc.relation.ispartof | PLoS Neglected Tropical Diseases, 2014, vol. 8, num. 1, p. e2640 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pntd.0002640 | - |
dc.rights | cc-by (c) Bisoffi, Zeno et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Malalties parasitàries | - |
dc.subject.classification | Larves | - |
dc.subject.classification | Serodiagnòstic | - |
dc.subject.other | Parasitic diseases | - |
dc.subject.other | Larvae | - |
dc.subject.other | Serodiagnosis | - |
dc.title | Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 704132 | - |
dc.date.updated | 2021-03-22T14:19:50Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/261495/EU//COHEMI | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24427320 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) |
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704132.pdf | 897.81 kB | Adobe PDF | View/Open |
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