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http://hdl.handle.net/2445/175693
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DC Field | Value | Language |
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dc.contributor.advisor | Fillat i Fonts, Cristina | - |
dc.contributor.author | Raimondi, Giulia | - |
dc.contributor.other | Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació | - |
dc.date.accessioned | 2021-03-24T11:19:29Z | - |
dc.date.available | 2021-06-08T05:10:20Z | - |
dc.date.issued | 2020-06-08 | - |
dc.identifier.uri | http://hdl.handle.net/2445/175693 | - |
dc.description.abstract | [eng] The general goal of this thesis has been to progress oncolytic adenovirus therapy for PDAC, by the incorporation of novel preclinical models to test for patient-specific responses and the generation of oncolytic adenoviruses with enhanced therapeutic index. The two main objectives have been the following: i) Evaluate patients-derived organoids (PDOs) technology as a platform to screen for personalized virotherapy in vitro 1) Establishment of a battery of PDOs from PDAC and normal pancreatic tissues, and evaluation of their applicability in the study of adenoviral infection; 2) Screening of a battery of PDOs to identify individual sensitivities to virotherapies, and the effects derived from the combination with chemotherapy; 3) Study virotherapy-responses in metastasis originated from PDOs xenografted in mice; (ii) Improve oncolytic adenovirus potency by modulation of miRNAs deregulated in PDAC 4) Screening of aberrantly expressed miRNAs sensitizing viral oncolysis in PDAC via CRISPR/Cas9 system; 5) Generation of a miRNA sponge-adenovirus and evaluation of its oncolytic effects in vitro and in vivo; 6) Modulation of miRNA levels with the THZ1 transcriptional inhibitor, and assessment of the effects of its combination with oncolytic adenoviruses. | ca |
dc.format.extent | 271 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | ca |
dc.publisher | Universitat de Barcelona | - |
dc.rights | cc by (c) Raimondi, Giulia, 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentació | - |
dc.subject.classification | Oncologia | - |
dc.subject.classification | Adenovirus | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.classification | Cultius cel·lulars humans | - |
dc.subject.other | Oncology | - |
dc.subject.other | Adenoviruses | - |
dc.subject.other | MicroRNAs | - |
dc.subject.other | Human cell culture | - |
dc.title | Broadening Adenoviral Oncolysis in PDAC: Interrogation of Patient-Derived Organoids for personalized virotherapy and modulation of miRNA content to boost adenoviral potency | ca |
dc.type | info:eu-repo/semantics/doctoralThesis | ca |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.tdx | http://hdl.handle.net/10803/671205 | - |
Appears in Collections: | Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentació |
Files in This Item:
File | Description | Size | Format | |
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GIULIA RAIMONDI_PhD_THESIS.pdf | 13.37 MB | Adobe PDF | View/Open |
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