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http://hdl.handle.net/2445/175743
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DC Field | Value | Language |
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dc.contributor.author | Lopez-Trujillo, Emilio | - |
dc.contributor.author | González Farré, Mónica | - |
dc.contributor.author | Pujol, Ramon M. | - |
dc.contributor.author | Bellosillo, Beatriz | - |
dc.contributor.author | Fisa Saladrigas, Roser | - |
dc.contributor.author | Riera Lizandra, Ma. Cristina | - |
dc.contributor.author | Alcover Amengual, Maria Magdalena | - |
dc.contributor.author | Barranco, Carlos | - |
dc.contributor.author | Martin-Ezquerra, Gemma | - |
dc.date.accessioned | 2021-03-25T10:55:53Z | - |
dc.date.issued | 2021-03-04 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/2445/175743 | - |
dc.description.abstract | Background: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness. Objectives: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis. Materials and methods: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results: From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, anti-CD1a and anti-Leishmania, was 94% [CI 95%: (79,8%-99,3%)] ; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. Conclusions: Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin - eosin and Giemsa staining. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Sercrisma International | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.14670/HH-18-324 | - |
dc.relation.ispartof | Histology and Histopathology, 2021 | - |
dc.relation.uri | https://doi.org/10.14670/HH-18-324 | - |
dc.rights | (c) Sercrisma International, 2021 | - |
dc.source | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) | - |
dc.subject.classification | Leishmània | - |
dc.subject.classification | Leishmaniosi | - |
dc.subject.classification | Antígens | - |
dc.subject.other | Leishmania | - |
dc.subject.other | Leishmaniasis | - |
dc.subject.other | Antigens | - |
dc.title | Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 707955 | - |
dc.date.updated | 2021-03-25T10:55:53Z | - |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | - |
dc.embargo.lift | 2026-03-04 | - |
dc.date.embargoEndDate | info:eu-repo/date/embargoEnd/2026-03-04 | - |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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File | Description | Size | Format | |
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707955.pdf | 1.14 MB | Adobe PDF | View/Open Request a copy |
Document embargat fins el 4-3-2026
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