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Title: Nystatin Regulates Axonal Extension and Regeneration by Modifying the Levels of Nitric Oxide
Author: Roselló Busquets, Cristina
Hernaiz Llorens, Marc
Soriano García, Eduardo
Martínez Mármol, Ramón
Keywords: Farmacologia
Medicaments antifúngics
Òxid nítric
Antifungal agents
Nitric oxide
Issue Date: 3-Apr-2020
Publisher: Frontiers Media
Abstract: Nystatin is a pharmacological agent commonly used for the treatment of oral, mucosal and cutaneous fungal infections. Nystatin has also been extensively applied to study the cellular function of cholesterol-enriched structures because of its ability to bind and extract cholesterol from mammalian membranes. In neurons, cholesterol level is tightly regulated, being essential for synapse and dendrite formation, and for axonal guidance. However, the action of Nystatin on axon regeneration has been poorly evaluated. Here, we examine the effect of Nystatin on primary cultures of hippocampal neurons, showing how acute dose (minutes) of Nystatin increases the area of growth cones, and chronic treatment (days) enhances axon length, axon branching and axon regeneration post-axotomy. We describe two alternative signaling pathways responsible for the observed effects, and activated at different concentrations of Nystatin. At elevated concentrations, Nystatin promotes growth cone expansion through phosphorylation of Akt; whereas at low concentrations, Nystatin enhances axon length and regrowth by increasing nitric oxide levels. Together, our findings indicate new signaling pathways of Nystatin and propose this compound as a novel regulator of axon regeneration.
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It is part of: Frontiers In Molecular Neuroscience, 2020, vol. 13
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ISSN: 1662-5099
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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