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Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
Author: Carvajal Romero, Silvia
Perramón, Meritxell
Casals, Gregori
Oró Bozzini, Denise
Ribera Sabaté, Jordi
Morales Ruiz, Manuel
Casals, Eudald
Casado, Pedro
Melgar Lesmes, Pedro
Fernández Varo, Guillermo
Cutillas, Pedro R.
Puntes, Víctor
Jiménez Povedano, Wladimiro
Keywords: Ceri (Element químic)
Estrès oxidatiu
Cèl·lules hepàtiques
Oxidative stress
Liver cells
Issue Date: 27-Nov-2019
Publisher: MDPI
Abstract: Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.
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It is part of: International Journal of Molecular Sciences, 2019, vol. 20, num. 23, p. 5959
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ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))
Articles publicats en revistes (Biomedicina)

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