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Title: Comparison of Molecular Recognition of Trimethyllysine and Trimethylthialysine by Epigenetic Reader Proteins
Author: Hintzen, Jordi C. J.
Poater i Teixidor, Jordi
Kumar, Kiran
Al Temimi, Abbas H. K.
Pieters, Bas J. G. E.
Paton, Robert S.
Bickelhaupt, F. Matthias
Mecinović, Jasmin
Keywords: Epigenètica
Issue Date: 21-Apr-2020
Publisher: MDPI
Abstract: Gaining a fundamental insight into the biomolecular recognition of posttranslationally modified histones by epigenetic reader proteins is of crucial importance to understanding the regulation of the activity of human genes. Here, we seek to establish whether trimethylthialysine, a simple trimethyllysine analogue generated through cysteine alkylation, is a good trimethyllysine mimic for studies on molecular recognition by reader proteins. Histone peptides bearing trimethylthialysine and trimethyllysine were examined for binding with five human reader proteins employing a combination of thermodynamic analyses, molecular dynamics simulations and quantum chemical analyses. Collectively, our experimental and computational findings reveal that trimethylthialysine and trimethyllysine exhibit very similar binding characteristics for the association with human reader proteins, thereby justifying the use of trimethylthialysine for studies aimed at dissecting the origin of biomolecular recognition in epigenetic processes that play important roles in human health and disease.
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It is part of: Molecules, 2020, vol. 25, num. 8, p. 1918
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ISSN: 1420-3049
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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