Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/176228
Title: | Integrated miRNA/mRNA counter-expression analysis highlights oxidative stress-related gnees CCR7 and FOX01 as blood markers of coronary arterial disease |
Author: | Hueso Val, Miguel Mallén, Adrián Casas, Ángela Guiteras, Jordi Sbraga, Fabrizio Blasco Lucas, Arnau Lloberas Blanch, Núria Torras Ambròs, Joan Cruzado, Josep Ma. Navarro, Estanis |
Keywords: | Estrès oxidatiu Arterioesclerosi Oxidative stress Arteriosclerosis |
Issue Date: | 2020 |
Publisher: | MDPI |
Abstract: | Our interest in the mechanisms of atherosclerosis progression (ATHp) has led to the recent identification of 13 miRNAs and 1285 mRNAs whose expression was altered during ATHp. Here, we deepen the functional relationship among these 13 miRNAs and genes associated to oxidative stress, a crucial step in the onset and progression of vascular disease. We first compiled a list of genes associated to the response to oxidative stress (Oxstress genes) by performing a reverse Gene Ontology analysis (rGO, from the GO terms to the genes) with the GO terms GO0006979, GO1902882, GO1902883 and GO1902884, which included a total of 417 unique Oxstress genes. Next, we identified 108 putative targets of the 13 miRNAs among these unique Oxstress genes, which were validated by an integrated miRNA/mRNA counter-expression analysis with the 1285 mRNAs that yielded 14 genes, Map2k1, Mapk1, Mapk9, Dapk1, Atp2a2, Gata4, Fos, Egfr, Foxo1, Ccr7, Vkorc1l1, Rnf7, Kcnh3, and Mgat3. GO enrichment analysis and a protein-protein-interaction network analysis (PPI) identified most of the validated Oxstress transcripts as components of signaling pathways, highlighting a role for MAP signaling in ATHp. Lastly, expression of these Oxstress transcripts was measured in PBMCs from patients suffering severe coronary artery disease, a serious consequence of ATHp. This allowed the identification of FOXO1 and CCR7 as blood markers downregulated in CAD. These results are discussed in the context of the interaction of the Oxstress transcripts with the ATHp-associated miRNAs. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms21061943 |
It is part of: | International Journal of Molecular Sciences, 2020, vol. 21, num. 6 |
URI: | http://hdl.handle.net/2445/176228 |
Related resource: | https://doi.org/10.3390/ijms21061943 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
710369.pdf | 1.55 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License