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Title: Generation of neurons from somatic cells of healthy individuals and neurological patients through induced pluripotency or direct conversion
Author: Velasco, Iván
Salazar, Patricia
Ramos-Mejía, Verónica
Castaño, Julio
Giorgetti, Alessandra
Romero Moya, Damià
Menéndez Buján, Pablo
Keywords: Cèl·lules
Enginyeria genètica
Genetic engineering
Issue Date: 1-Nov-2014
Publisher: AlphaMed Press
Abstract: Access to healthy or diseased human neural tissue is a daunting task and represents a barrierfor advancing our understanding about the cellular, genetic, and molecular mechanisms underly-ing neurogenesis and neurodegeneration. Reprogramming of somatic cells to pluripotency bytransient expression of transcription factors was achieved a few years ago. Induced pluripotentstem cells (iPSC) from both healthy individuals and patients suffering from debilitating, life-threatening neurological diseases have been differentiated into several specific neuronal sub-types. An alternative emerging approach is the direct conversion of somatic cells (i.e., fibro-blasts, blood cells, or glial cells) into neuron-like cells. However, to what extent neuronal directconversion of diseased somatic cells can be achieved remains an open question. Optimizationof current expansion and differentiation approaches is highly demanded to increase the differ-entiation efficiency of specific phenotypes of functional neurons from iPSCs or through somaticcell direct conversion. The realization of the full potential of iPSCs relies on the ability to pre-cisely modify specific genome sequences. Genome editing technologies including zinc fingernucleases, transcription activator-like effector nucleases, and clustered regularly interspacedshort palindromic repeat/CAS9 RNA-guided nucleases have progressed very fast over the lastyears. The combination of genome-editing strategies and patient-specific iPSC biology will offera unique platform for in vitro generation of diseased and corrected neural derivatives for per-sonalized therapies, disease modeling and drug screening.
Note: Versió postprint del document publicat a:
It is part of: Stem Cells, 2014, vol. 32, num. 11, p. 2811-2817
Related resource:
ISSN: 1066-5099
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Biomedicina)

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