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Title: | Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2 |
Author: | Giorgetti, Alessandra Montserrat, Núria Aasen, Trond Gonzalez, Federico Rodríguez-Pizà, Ignacio Vassena, Rita Raya Chamorro, Ángel Boué, Stefanie Barrero, Maria J. Aran Corbella, Begoña Torrabadella, Marta Veiga, Anna Izpisúa Belmonte, Juan Carlos |
Keywords: | Cèl·lules mare Cordó umbilical Citologia Stem cells Umbilical cord Cytology |
Issue Date: | 2-Oct-2009 |
Publisher: | Elsevier B.V. |
Abstract: | Mouse and human fibroblasts were the first cell types successfully reprog- rammed by ectopic expression of OCT4, SOX2, KLF4, and c-MYC (OSKM) (Lowry et al., 2008; Maherali et al., 2007; Park et al., 2008; Takahashi et al., 2007; Taka- hashi and Yamanaka, 2006; Yu et al., 2007). Further studies have shown that the age, origin, and cell type used have a deep impact on the reprogramming effi- ciency, eventually requiring the expres- sion of fewer factors and/or reducing the timing of the whole process. In general, stem cells are rare and difficult to access and isolate in large numbers (neural stem cells, for instance [Kim et al., 2008, 2009c]) and, therefore, represent a com- plicated target for reprogramming. How- ever, Cord Blood (CB) could represent an alternative and readily accessible source of stem cells. Here, we describe reprog- ramming of CB cells to pluripotency by retroviral transduction of four (OSKM), three (OSK), and as few as two (OS) tran- scription factors, without the need for additional chemical compounds. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.stem.2009.09.008 |
It is part of: | Cell Stem Cell, 2009, vol. 5, num. 4, p. 353-357 |
URI: | http://hdl.handle.net/2445/176410 |
Related resource: | https://doi.org/10.1016/j.stem.2009.09.008 |
ISSN: | 1934-5909 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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711108.pdf | 1.26 MB | Adobe PDF | View/Open |
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