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http://hdl.handle.net/2445/176426
Title: | Unveiling Viruses Associated with Gastroenteritis Using a Metagenomics Approach |
Author: | Fernández Cassi, Xavier Martínez-Puchol, Sandra Silva Sales, Marcelle Cornejo Sánchez, Thais Bartolomé, Rosa Bofill Mas, Silvia Gironès Llop, Rosina |
Keywords: | Gastroenteritis Infants Metagenòmica Gastroenteritis Children Metagenomics |
Issue Date: | 13-Dec-2020 |
Publisher: | MDPI |
Abstract: | Acute infectious gastroenteritis is an important illness worldwide, especially on children, with viruses accounting for approximately 70% of the acute cases. A high number of these cases have an unknown etiological agent and the rise of next generation sequencing technologies has opened new opportunities for viral pathogen detection and discovery. Viral metagenomics in routine clinical settings has the potential to identify unexpected or novel variants of viral pathogens that cause gastroenteritis. In this study, 124 samples from acute gastroenteritis patients from 2012-2014 previously tested negative for common gastroenteritis pathogens were pooled by age and analyzed by next generation sequencing (NGS) to elucidate unidentified viral infections. The most abundant sequences detected potentially associated to acute gastroenteritis were from Astroviridae and Caliciviridae families, with the detection of norovirus GIV and sapoviruses. Lower number of contigs associated to rotaviruses were detected. As expected, other viruses that may be associated to gastroenteritis but also produce persistent infections in the gut were identified including several Picornaviridae members (EV, parechoviruses, cardioviruses) and adenoviruses. According to the sequencing data, astroviruses, sapoviruses and NoV GIV should be added to the list of viral pathogens screened in routine clinical analysis. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/v12121432 |
It is part of: | Viruses, 2020, vol. 12, num. 12 |
URI: | http://hdl.handle.net/2445/176426 |
Related resource: | https://doi.org/10.3390/v12121432 |
ISSN: | 1999-4915 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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