Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/176584
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Martínez Val, Ana | - |
dc.contributor.author | Lynch, Cian J. | - |
dc.contributor.author | Calvo Serrano, Isabel | - |
dc.contributor.author | Ximénez Embún, Pilar | - |
dc.contributor.author | García, Fernando | - |
dc.contributor.author | Serrano Marugán, Manuel | - |
dc.contributor.author | Zarzuela, Eduardo | - |
dc.contributor.author | Muñoz, Javier | - |
dc.date.accessioned | 2021-04-26T07:01:51Z | - |
dc.date.available | 2021-04-26T07:01:51Z | - |
dc.date.issued | 2021-12-01 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176584 | - |
dc.description.abstract | Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimulates enhancers and, similar to 2i, stabilizes ESCs in the naïve state. Here, we use mass spectrometry to describe the molecular events (phosphoproteome, proteome, and metabolome) triggered by 2i and Cdk8/19i on ESCs. Our data reveal widespread commonalities between these two treatments, suggesting overlapping processes. We find that post-transcriptional de-repression by both 2i and Cdk8/19i might support the mitochondrial capacity of naive cells. However, proteome reprogramming in each treatment is achieved by different mechanisms. Cdk8/19i acts directly on the transcriptional machinery, activating key identity genes to promote the naïve program. In contrast, 2i stabilizes the naïve circuitry through, in part, de-phosphorylation of downstream transcriptional effectors. | - |
dc.format.extent | 16 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Research | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41467-021-22181-5 | - |
dc.relation.ispartof | Nature Communications, 2021, vol. 12 | - |
dc.relation.uri | https://doi.org/10.1038/s41467-021-22181-5 | - |
dc.rights | cc by (c) Martínez Val et al., 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) | - |
dc.subject.classification | Cèl·lules mare embrionàries | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.other | Embryonic stem cells | - |
dc.subject.other | Mass spectrometry | - |
dc.title | Dissection of two routes to naïve pluripotency using different kinase inhibitors | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2021-04-20T08:00:50Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/669622/EU//CELLPLASTICITY | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 6485916 | - |
dc.identifier.pmid | 33767186 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
12541_6485916_martinez-val_et_al_natcommun_2021.pdf | 3.14 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License