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Title: Cord blood-derived neuronal cells by ectopic expression of SOX2 and c-MYC
Author: Giorgetti, Alessandra
Marchetto, Maria C.N.
Li, Mo
Yu, Diana
Fazzina, Raffaella
Mu, Yangling
Adamo, Antonio
Paramonov, Ida
Castaño, Julio
Barragan Monasterio, Montserrat
Bardy, Cedric
Cassiani-Ingoni, Riccardo
Liu, Guang-Hui
Gage, Fred H.
Izpisúa Belmonte, Juan Carlos
Keywords: Cordó umbilical
Embaràs extrauterí
Umbilical cord
Ectopic pregnancy
Issue Date: 31-Jul-2012
Publisher: National Academy of Sciences
Abstract: The finding that certain somatic cells can be directly converted into cells of other lineages by the delivery of specific sets of transcrip- tion factors paves the way to novel therapeutic applications. Here we show that human cord blood (CB) CD133+ cells lose their hematopoietic signature and are converted into CB-induced neu- ronal-like cells (CB-iNCs) by the ectopic expression of the transcrip- tion factor Sox2, a process that is further augmented by the combination of Sox2 and c-Myc. Gene-expression analysis, immu- nophenotyping, and electrophysiological analysis show that CB- iNCs acquire a distinct neuronal phenotype characterized by the expression of multiple neuronal markers. CB-iNCs show the ability to fire action potentials after in vitro maturation as well as after in vivo transplantation into the mouse hippocampus. This system highlights the potential of CB cells and offers an alternative means to the study of cellular plasticity, possibly in the context of drug screening research and of future cell-replacement therapies.
Note: Reproducció del document publicat a:
It is part of: Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2012, vol. 109, num. 31, p. 12556-12561
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ISSN: 0027-8424
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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