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http://hdl.handle.net/2445/176737
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DC Field | Value | Language |
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dc.contributor.author | Mora Fayos, Sílvia | - |
dc.contributor.author | Durham, Paul L. | - |
dc.contributor.author | Smith, Jeffery R. | - |
dc.contributor.author | Russo, Andrew F. | - |
dc.contributor.author | Jeromin, Andrea | - |
dc.contributor.author | Pessin, Jeffrey E. | - |
dc.date.accessioned | 2021-04-26T14:12:25Z | - |
dc.date.available | 2021-04-26T14:12:25Z | - |
dc.date.issued | 2002-07-26 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176737 | - |
dc.description.abstract | Expression of NCS-1 (neuronal calcium sensor-1, also termed frequenin) in 3T3L1 adipocytes strongly inhibited insulin-stimulated translocation of GLUT4 and insulin-responsive aminopeptidase. The effect of NCS-1 was specific for GLUT4 and the insulin-responsive aminopeptidase translocation as there was no effect on the trafficking of the cation-independent mannose 6-phosphate receptor or the GLUT1 glucose transporter isoform. Moreover, NCS-1 showed partial colocalization with GLUT4-EGFP in the perinuclear region. The inhibitory action of NCS-1 was independent of calcium sequestration since neither treatment with ionomycin nor endothelin-1, both of which elevated the intracellular calcium concentration, restored insulin-stimulated GLUT4 translocation. Furthermore, NCS-1 did not alter the insulin-stimulated protein kinase B (PKB/Akt) phosphorylation or the recruitment of Cbl to the plasma membrane. In contrast, expression of the NCS-1 effector phosphatidylinositol 4-kinase (PI 4-kinase) inhibited insulin-stimulated GLUT4 translocation, whereas co-transfection with an inactive PI 4-kinase mutant prevented the NCS-1-induced inhibition. These data demonstrate that PI 4-kinase functions to negatively regulate GLUT4 translocation through its interaction with NCS-1. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M203669200 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 2002, vol. 277, p. 27494-27500 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.M203669200 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 2002 | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Fosforilació | - |
dc.subject.classification | Teixit adipós | - |
dc.subject.classification | Receptors d'insulina | - |
dc.subject.other | Phosphorylation | - |
dc.subject.other | Adipose tissues | - |
dc.subject.other | Insulin receptors | - |
dc.title | NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 711519 | - |
dc.date.updated | 2021-04-26T14:12:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
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711519.pdf | 748.76 kB | Adobe PDF | View/Open |
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