Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/176796
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dc.contributor.authorFont Nieves de La Vega, Miriam Aurora-
dc.contributor.authorSans Fons, Gloria-
dc.contributor.authorGorina Méndez, Roser-
dc.contributor.authorBonfill-Teixidor, Ester-
dc.contributor.authorSalas Perdomo, Angélica María-
dc.contributor.authorMárquez-Kisinousky, Leonardo-
dc.contributor.authorSantalucía Albi, Tomàs-
dc.contributor.authorPlanas Obradors, Anna Maria-
dc.date.accessioned2021-04-27T14:35:56Z-
dc.date.available2021-04-27T14:35:56Z-
dc.date.issued2012-02-24-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/2445/176796-
dc.description.abstractPathological conditions and pro-inflammatory stimuli in the brain induce cyclooxygenase-2 (COX-2), a key enzyme in arachidonic acid metabolism mediating the production of prostanoids that, among other actions, have strong vasoactive properties. Although low basal cerebral COX-2 expression has been reported, COX-2 is strongly induced by pro-inflammatory challenges, whereas COX-1 is constitutively expressed. However, the contribution of these enzymes in prostanoid formation varies depending on the stimuli and cell type. Astrocyte feet surround cerebral microvessels and release molecules that can trigger vascular responses. Here, we investigate the regulation of COX-2 induction and its role in prostanoid generation after a pro-inflammatory challenge with the bacterial lipopolysaccharide (LPS) in astroglia. Intracerebral administration of LPS in rodents induced strong COX-2 expression mainly in astroglia and microglia, whereas COX-1 expression was predominant in microglia and did not increase. In cultured astrocytes, LPS strongly induced COX-2 and microsomal prostaglandin-E2 (PGE2) synthase-1, mediated by the MyD88-dependent NFκB pathway and influenced by mitogen-activated protein kinase pathways. Studies in COX-deficient cells and using COX inhibitors demonstrated that COX-2 mediated the high production of PGE2 and, to a lesser extent, other prostanoids after LPS. In contrast, LPS down-regulated COX-1 in an MyD88-dependent fashion, and COX-1 deficiency increased PGE2 production after LPS. The results show that astrocytes respond to LPS by a COX-2-dependent production of prostanoids, mainly vasoactive PGE2, and suggest that the coordinated down-regulation of COX-1 facilitates PGE2 production after TLR-4 activation. These effects might induce cerebral blood flow responses to brain inflammation.-
dc.format.extent15 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1074/jbc.M111.327874-
dc.relation.ispartofJournal of Biological Chemistry, 2012, vol. 287, num. 9, p. 6454-6468-
dc.relation.urihttps://doi.org/10.1074/jbc.M111.327874-
dc.rights(c) American Society for Biochemistry and Molecular Biology, 2012-
dc.sourceArticles publicats en revistes (Infermeria Fonamental i Clínica)-
dc.subject.classificationAstròcits-
dc.subject.classificationEnzimologia-
dc.subject.classificationMetabolisme-
dc.subject.classificationProteïnes de membrana-
dc.subject.otherAstrocytes-
dc.subject.otherEnzymology-
dc.subject.otherMetabolism-
dc.subject.otherMembrane proteins-
dc.titleInduction of COX-2 enzyme and down-regulation of COX-1 expression by lipopolysaccharide (LPS) control prostaglandin E2 production in astrocytes-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec660981-
dc.date.updated2021-04-27T14:35:56Z-
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/201024/EU//ARISE-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid22219191-
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Infermeria Fonamental i Clínica)
Articles publicats en revistes (Medicina)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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