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Title: Micro-computed tomography and histology to explore internal morphology in decapod larvae
Author: Castejón Bueno, Diego
Alba Tercedor, Javier
Rotllant, Guiomar
Ribes Mora, Enric
Durfort i Coll, Mercè
Guerao, Guillermo
Keywords: Larves
Sistema nerviós
Nervous system
Issue Date: 26-Sep-2018
Publisher: Nature Publishing Group
Abstract: Traditionally, the internal morphology of crustacean larvae has been studied using destructive techniques such as dissection and microscopy. The present study combines advances in micro-computed tomography (micro-CT) and histology to study the internal morphology of decapod larvae, using the common spider crab (Maja brachydactyla Balss, 1922) as a model and resolving the individual limitations of these techniques. The synergy of micro-CT and histology allows the organs to be easily identified, revealing simultaneously the gross morphology (shape, size, and location) and histological organization (tissue arrangement and cell identification). Micro-CT shows mainly the exoskeleton, musculature, digestive and nervous systems, and secondarily the circulatory and respiratory systems, while histology distinguishes several cell types and confirms the organ identity. Micro-CT resolves a discrepancy in the literature regarding the nervous system of crab larvae. The major changes occur in the metamorphosis to the megalopa stage, specifically the formation of the gastric mill, the shortening of the abdominal nerve cord, the curving of the abdomen beneath the cephalothorax, and the development of functional pereiopods, pleopods, and lamellate gills. The combination of micro-CT and histology provides better results than either one alone.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2018, vol. 8, num. 1, p. 14399
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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