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Title: Amino acids activate mammalian target of rapamycin Complex 2 (mTORC2) via PI3K/Akt signaling
Author: Tato, Irantzu
Bartrons Bach, Ramon
Ventura Pujol, Francesc
Rosa López, José Luis
Keywords: Aminoàcids
Transducció de senyal cel·lular
Factors de transcripció
Amino acids
Cellular signal transduction
Transcription factors
Issue Date: 25-Feb-2011
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: The activity of mammalian target of rapamycin (mTOR) complexes regulates essential cellular processes, such as growth, proliferation, or survival. Nutrients such as amino acids are important regulators of mTOR complex 1 (mTORC1) activation, thus affecting cell growth, protein synthesis, and autophagy. Here, we show that amino acids may also activate mTOR complex 2 (mTORC2). This activation is mediated by the activity of class I PI3K and of Akt. Amino acids induced a rapid phosphorylation of Akt at Thr-308 and Ser-473. Whereas both phosphorylations were dependent on the presence of mTOR, only Akt phosphorylation at Ser-473 was dependent on the presence of rictor, a specific component of mTORC2. Kinase assays confirmed mTORC2 activation by amino acids. This signaling was functional, as demonstrated by the phosphorylation of Akt substrate FOXO3a. Interestingly, using different starvation conditions, amino acids can selectively activate mTORC1 or mTORC2. These findings identify a new signaling pathway used by amino acids underscoring the crucial importance of these nutrients in cell metabolism and offering new mechanistic insights.
Note: Reproducció del document publicat a:
It is part of: Journal of Biological Chemistry, 2011, vol. 286, num. 8, p. 6128-6142
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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