Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/176903
Title: | Extracellular vesicles from hypoxic adipocytes and obese subjects reduce insulin-stimulated glucose uptake |
Author: | Mleczko, Justyna Ortega, Francisco J. Falcon Perez, Juan Manuel Wabitsch, Martin Fernandez-Real, Jose Manuel Mora Fayos, Sílvia |
Keywords: | Teixit adipós Glucosa Insulina Adipose tissues Glucose Insulin |
Issue Date: | 2018 |
Publisher: | Wiley-VCH |
Abstract: | Scope We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin‐stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin‐stimulated 2‐deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin‐mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2‐deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion EVs released by stressed adipocytes impair insulin action in neighboring adipocytes. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1002/mnfr.201700917 |
It is part of: | Molecular Nutrition & Food Research, 2018, vol. 62, p. 1700917 |
URI: | http://hdl.handle.net/2445/176903 |
Related resource: | https://doi.org/10.1002/mnfr.201700917 |
ISSN: | 1613-4125 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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File | Description | Size | Format | |
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709807.pdf | 1.89 MB | Adobe PDF | View/Open |
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