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Title: | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
Author: | Millares, Laura Pascual, Sergi Montón, Concepción García Núñez, Marian Lalmolda, Cristina Faner, Rosa Casadevall, Carme Setó, Laia Capilla, Silvia Moreno, Amàlia Castro Acosta, Ady Angélica Álvarez Martínez, Carlos José Sibila Vidal, Oriol Peces Barb, Germán Cosío, Borja G. Agustí García-Navarro, Àlvar Gea Guiral, Joaquim Monsó, Eduard |
Keywords: | Microbiota Pseudomonas Microbiota Pseudomonas |
Issue Date: | 24-Jun-2019 |
Publisher: | BioMed Central |
Abstract: | Background: The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods: Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results: We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. Conclusion: The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels. |
Note: | Reproducció del document publicat a: https://doi.org/10.1186/s12890-019-0867-x |
It is part of: | BMC Pulmonary Medicine, 2019, vol. 19, num. 1, p. 112 |
URI: | http://hdl.handle.net/2445/177071 |
Related resource: | https://doi.org/10.1186/s12890-019-0867-x |
ISSN: | 1471-2466 |
Appears in Collections: | Articles publicats en revistes (Medicina) |
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